Limited Edition Golden Llama is here! Check out how you can get one. 
Limited Edition Golden Llama is here! Check out how you can get one.
Offering SPR-BLI Services - Proteins provided for free! Offering SPR-BLI Services - Proteins provided for free!
Thank you for choosing ACROBiosystems. Would you rate our product and service？ Thank you for choosing ACROBiosystems. Would you rate our product and service？
Here come GMP Grade Cytokines！Free Sample is available！ Here come GMP Grade Cytokines！Free Sample is available！
A-Z
> ノックアウト細胞株
遺伝子機能研究 [1]
ターゲットとトランスダクション研究
疾患のメカニズム研究 [2]
分子標的薬研究 [3]
創薬とスクリーニング
医薬品の安全性評価
発現解析（FACS）とゲノム配列決定により検証され、遺伝子ノックアウトの正確性と信頼性が保証された。
遺伝子組み換え細胞株はMOA（作用機序）を最もよく反映する
堅牢で再現性の高い細胞ベースのバイオアッセイのための、より高い活性と大きなアッセイウィンドウ
アッセイ開発とバリデーションをサポートする包括的なアプリケーションデータ
完全な追跡可能記録、厳格な品質管理、有効な細胞継代安定性
親細胞株は国際的に認知された細胞資源バンクから合法的に入手し、商業ライセンスを取得した
規制当局への申請が必要な場合はいつでも、グローバルな商業ライセンス支援。
当社の確立された 遺伝子編集プラットフォームにより、
お客様の特定のターゲットに合わせてカスタマイズされた遺伝子ADC Targeting & CAR-T Cell Therapy
| Molecule | Cat. No. | Product Description | Order/Pre-order |
|---|---|---|---|
| CD19 | SCRAJ-STT216 | Raji/Human CD19 Knockout Stable Cell Line |
All of our cell line products are monoclonal cell lines.
According to our current company policy:
- With a signed Non-Disclosure Agreement (NDA), we are authorized to share the vector map.
- Without a signed NDA: We may disclose the sequence of the overexpressed target gene. Additionally, we can provide lentiviral residual testing reports upon request.
The following confidential information is not disclosed:
1. The complete vector sequence and the original vector name.
2. Sequences of signal response elements related to signal transduction pathways.
Cell lines are shipped on dry ice. To ensure optimal cell viability, we recommend thawing and initiating culture immediately upon receipt. If immediate thawing and culturing are not possible, we advise transferring the cells to liquid nitrogen for long-term storage. Please ensure the transfer process is quick to avoid thawing, as this may impact the long-term stability and viability of the cells.
If immediate transfer to liquid nitrogen is not feasible, the cells can be temporarily stored in a -80°C freezer. However, we recommend that the storage period from the date of receipt should not exceed two weeks. Long-term storage on dry ice or in a -80°C freezer is not recommended.
When using the cells, please refer to the recommended thawing and culturing methods provided in the DS.
Please Note: If the cells are received unfrozen or not on dry ice, please contact our technical support team immediately at techsupport@acrobiosystems.com.
We provide two cryovials of cell lines to ensure the smooth progress of your experiments. In the event of any issues with thawing, recovery, or culturing of the first vial, please contact our technical support team (techsupport@acrobiosystems.com) for troubleshooting before thawing the second vial.
All ACRO cell lines undergo pre-shipment validation for recovery and culturing. Additionally, we recommend establishing a cell bank at the earliest possible passage stage to ensure long-term use.
After thawing the cells, they should initially be cultured in a medium without selection antibiotics for 1-2 passages. If the cells exhibit good condition, you can switch to a medium with selection antibiotics for further passaging. For guidance on selecting appropriate antibiotics, please refer to FAQ12.
Additionally, we recommend adding P/S (Penicillin-Streptomycin) to the culture medium throughout the entire cell culture process to maintain aseptic conditions.
For Adherent Cells (e.g., HEK293):
• Confluence: Avoid over-confluence during culture. If the confluence is too high (exceeding 100%), it may significantly affect cell viability after passaging. Please refer to the passaging methods and precautions in the DS for specific instructions.
• Post-Passaging Issues If cells exhibit poor adherence after passaging due to over-confluence or other reasons, we recommend:
- Removing selection antibiotics from the culture medium.
- Passaging at a higher cell density (e.g., 1×107 cells per T75 flask).
- Resuming normal passaging only after cell viability has recovered.
For Suspension Cells (e.g., Jurkat and Raji):
• Cell Density: Avoid excessively high cell density. If the density is too high (exceeding 3×106cells/mL), it may significantly affect cell viability after passaging. Please refer to the passaging methods and precautions in the DS for specific instructions.
• Post-Passaging Issues: If cells exhibit poor viability after passaging due to high density or other reasons, we recommend:
- Removing selection antibiotics from the culture medium.
- Passaging at a lower density (e.g., 1×105-2×105 cells/mL).
- Resuming normal passaging only after cell viability has recovered.
Always monitor cell health and adjust protocols as needed to maintain optimal growth conditions.
We recommend starting with a transparent 96-well plate for cell seeding (refer to the specific experimental protocol for details). This allows for easy observation of cell status and determination of whether the cell density is appropriate. Once the experimental conditions are optimized, you can transition to other suitable culture plates.
For recommended cell seeding densities, please refer to FAQ9.
For a list of commonly used culture plates and consumables, see the product experimental protocol.
No, it is not necessary to add selection antibiotics.
For 96-well plates, we recommend seeding cells such that they reach approximately 80% confluence after overnight culture before conducting functional experiments. You can start by following the cell seeding density recommended in the ACRO experimental protocol or by testing a gradient of different cell densities to determine the optimal conditions for your experimental system.
During the initial stages of functional experiments, you can start by testing the protein or drug concentrations recommended by ACRO. However, due to variations in reagents or experimental conditions, we recommend conducting preliminary optimization experiments to determine the optimal concentration best suited for your specific experimental system.
We recommend prioritizing the use of culture media and serum from the manufacturers specified in the DS. However, you may also choose to use comparable alternatives or other suitable culture media and serum (e.g., Gibco) for testing and cultivation.
For selection antibiotics, we highly recommend using the brands specified in the DS. The activity of antibiotics may vary between manufacturers, so if you choose to use a different brand, it is essential to validate whether the concentration recommended in the ACRO culture protocol is suitable.
Regardless of the brand used, we recommend maintaining a backup culture without selection antibiotics to avoid potential cell loss due to inappropriate antibiotic concentration.
We highly recommend using the protein reagents from the manufacturers specified in the experimental protocol, as their activity has been validated by us. If you choose to use protein products from other manufacturers, we recommend conducting a concentration optimization based on the recommended concentrations in the protocol to identify the appropriate concentration for your experiments.
For both adherent and suspension cells, we recommend using 90% FBS + 10% DMSO (V/V) as the freezing medium. Alternatively, you may choose commercial cell freezing media or other suitable freezing media commonly used in your laboratory.
Recommended freezing density: 5×106 - 1×107cells/mL.
[1]. Nieuwe methode om regulatie van genen in individuele cellen te bestuderen. Hubrecht Institute. Published 2019 Jun 17.
[2]. Plundrich D, Chikhladze S, Fichtner-Feigl S, Feuerstein R, Briquez PS. Molecular Mechanisms of Tumor Immunomodulation in the Microenvironment of Colorectal Cancer. Int J Mol Sci. 2022;23(5):2782. Published 2022 Mar 3. doi:10.3390/ijms23052782
[3]. Zhang DKY, Cheung AS, Mooney DJ. Activation and expansion of human T cells using artificial antigen-presenting cell scaffolds. Nat Protoc. 2020;15(3):773-798. doi:10.1038/s41596-019-0249-0
This web search service is supported by Google Inc.
アクロバイオシステムズの公式アカウント
