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218 linker

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218-AFFMY3-Cell-based assay
 218 linker FACS

Flow cytometric analysis of Anti-BCMA (C11D5.3) CAR 293 cells staining with Alexa Fluor 488-Labeled Monoclonal Anti-Whitlow 218 linker Antibody, Rabbit IgG (P3C09) (0.03% Proclin) (Cat. No. 218-AFFMY3) at 1:25 dilution (4 μL of the antibody stock solution corresponds to labeling of 1e6 cells in a final volume of 100 µL). Alexa Fluor 488 signal was used to evaluate the binding activity (QC tested).

218-AFFMY3-Cell-based assay
 218 linker FACS

Non-specificity of Alexa Fluor 488-Labeled Monoclonal Anti-Whitlow 218 linker Antibody, Rabbit IgG (P3C09) (0.03% Proclin) (Cat. No. 218-AFFMY3) binding to CD3+ cells present in human PBMC. 5e5 of human PBMCs were simultaneously stained with PE-labeled anti-CD3 antibody and Alexa Fluor 488-Labeled Monoclonal Anti-Whitlow 218 linker Antibody (4 μL of the antibody stock solution corresponds to labeling of 5e5 cells in a final volume of 100 µL) and washed and then analyzed with FACS. Both Alexa Fluor 488 and PE positive signals was used to evaluate the non-specific binding activity to human CD3+ cells (QC tested).

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