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Your Position: ホーム > Protein > SLAMF7 > SL7-H82E0

Biotinylated Human SLAMF7 / CRACC / CD319 Protein, Avitag™,His Tag (HPLC verified)

  • Synonym
    SLAMF7,CD319,CS1,CRACC,19A,FOAP-12
  • Source
    Biotinylated Human SLAMF7, Avitag,His Tag(SL7-H82E0) is expressed from human 293 cells (HEK293). It contains AA Ser 23 - Met 226 (Accession # Q9NQ25-1).
    Predicted N-terminus: Ser 23
  • Molecular Characterization
    SLAMF7 Structure

    This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag

    The protein has a calculated MW of 25.6 kDa. The protein migrates as 40-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-HPLC.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
SLAMF7 SDS-PAGE

Biotinylated Human SLAMF7, Avitag,His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-HPLC
SLAMF7 SEC-HPLC

The purity of Biotinylated Human SLAMF7, Avitag,His Tag (Cat. No. SL7-H82E0) was greater than 90% as determined by SEC-HPLC.

Bioactivity-ELISA
 SLAMF7 ELISA

Immobilized Anti-SLAMF7/CS1 Antibody, Human IgG1 at 1 μg/mL (100 μL/well) can bind Biotinylated Human SLAMF7, Avitag,His Tag (Cat. No. SL7-H82E0) with a linear range of 0.2-2 ng/mL (QC tested).

 SLAMF7 ELISA

Immobilized Humanized Anti-Human CS1 MAb at 1 μg/mL (100 μL/well) can bind Biotinylated Human SLAMF7, Avitag,His Tag (Cat. No. SL7-H82E0) with a linear range of 0.6-5 ng/mL (Routinely tested).

Bioactivity-FACS
 SLAMF7 FACS

2e5 of Anti-SLAMF7 CAR-293 cells were stained with 100 μL of 0.1 μg/mL of Biotinylated Human SLAMF7, Avitag, His Tag (Cat. No. SL7-H82E0) and negative control protein respectively, washed and then followed by PE-SA and analyzed with FACS (QC tested).

  • Background
    SLAM family member 7 (SLAMF7) is also known as CD2-like receptor-activating cytotoxic cells (CRACC), Membrane protein FOAP-12, CD antigen CD319, Novel Ly9, Protein 19A, which is a single-pass type I membrane protein and a member of the CD2 family of cell surface receptors. SLAMF7 is expressed in spleen, lymph node, peripheral blood leukocytes, bone marrow, small intestine, stomach, appendix, lung and trachea. Isoform 1 of SLAMF7 mediates NK cell activation through a SH2D1A-independent extracellular signal-regulated ERK-mediated pathway. May play a role in lymphocyte adhesion. Isoform 3 of SLAMF7 does not mediate any NK cell activation.
  • Clinical and Translational Updates

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