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Your Position: ホーム > Protein > NSE > NSE-H5144

Human NSE Protein, His Tag (MALS verified)

  • Synonym
    Neuron-specific enolase,gamma-enolase
  • Source
    Human NSE, His Tag(NSE-H5144) is expressed from E. coli cells. It contains AA Met 1 - Leu 434 (Accession # P09104-1).
    Predicted N-terminus: Met
  • Molecular Characterization
    NSE Structure

    This protein carries a polyhistidine tag at the N-terminus

    The protein has a calculated MW of 49.3 kDa. The protein migrates as 46-50 kDa under reducing (R) condition (SDS-PAGE).

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 20 mM Tris, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
NSE SDS-PAGE

Human NSE, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
NSE MALS images

The purity of Human NSE, His Tag (Cat. No. NSE-H5144) is more than 90% and the molecular weight of this protein is around 85-110 kDa verified by SEC-MALS.

Bioactivity-ELISA
 NSE ELISA

Immobilized Human NSE, His Tag (Cat. No. NSE-H5144) at 1 μg/mL (100 μL/well) can bind Anti-NSE antibody with a linear range of 1-63 ng/mL (QC tested).

  • Background
    Neuron-specific enolase (NSE) is known to be a cell specific isoenzyme of the glycolytic enzyme enolase. It is also known as
    gamma-enolase or nolase 2 (ENO2). It has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. NSE is a highly specific marker for neurons and peripheral neuroendocrine cells. In clinical, NSE could be used as a biomarker for neuronal injury.
  • Clinical and Translational Updates

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