Biotinylated Monoclonal Anti-G4S linker Antibody, Rabbit IgG (016), premium grade

Immobilized Recombinant Protein containing (G4S)2, Recombinant Protein containing (G4S)3 at 1 μg/mL (100 μL/well) can bind Biotinylated Monoclonal Anti-G4S linker Antibody, Rabbit IgG (016), premium grade (Cat. No. G4S-BLM25G0) with a linear range of 0.1-8 ng/mL (Routinely tested).

Immobilized Recombinant Protein containing (G3S)2, Recombinant Protein containing (G3S)3, Recombinant Protein containing (G3S)4 at 1 μg/mL (100 μL/well) can bind Biotinylated Monoclonal Anti-G4S linker Antibody, Rabbit IgG (016), premium grade (Cat. No. G4S-BLM25G0) with a linear range of 0.1-31 ng/mL (Routinely tested).

Flow cytometric analysis of Anti-MSLN CAR-293 cells staining with Biotinylated Monoclonal Anti-G4S linker Antibody, Rabbit IgG (016), premium grade (Cat. No. G4S-BLM25G0) at 3 µg/mL (0.3 µg corresponds to labeling of 1e6 cells in a final volume of 100 µL) and PE-SA. PE signal was used to evaluate the binding activity (QC tested).

Non-specificity of Biotinylated Monoclonal Anti-G4S linker Antibody, Rabbit IgG (016), premium grade (Cat. No. G4S-BLM25G0) binding to CD3+ cells present in human PBMC. 5e5 of human PBMCs were simultaneously stained with FITC-labeled anti-CD3 antibody and Biotinylated Monoclonal Anti-G4S linker Antibody, Rabbit IgG (016), premium grade (0.3 µg corresponds to labeling of 5e5 cells in a final volume of 100 µL) and washed and then analyzed with FACS. Both FITC and PE positive signals was used to evaluate the non-specific binding activity to human CD3+ cells (QC tested).

Flow cytometric analysis of Anti-CD22 CAR-293 cells staining with Biotinylated Monoclonal Anti-G4S linker Antibody, Rabbit IgG (016), premium grade (Cat. No. G4S-BLM25G0) at 3 µg/mL (0.3 µg corresponds to labeling of 1e6 cells in a final volume of 100 µL) and PE-SA. PE signal was used to evaluate the binding activity (Routinely tested).

Flow cytometric analysis of Anti-CD19 CAR-293 cells staining with Biotinylated Monoclonal Anti-G4S linker Antibody, Rabbit IgG (016), premium grade (Cat. No. G4S-BLM25G0) at 3 µg/mL (0.3 µg corresponds to labeling of 1e6 cells in a final volume of 100 µL) and PE-SA. PE signal was used to evaluate the binding activity (Routinely tested).

The Isolation of Anti-CD19 CAR-T cells with Biotinylated Anti-G4S Linker Antibody and SA Nanobeads. Each 1E7 Anti-CD19 CAR-T cells were isolated with 0.6 µg Biotinylated Monoclonal Anti-G4S linker Antibody, Rabbit IgG (016), premium grade (Cat. No. G4S-BLM25G0) and appropriate SA Nanobeads. Both the isolated cells and non-isolated cells were stained with Alexa Fluor 647-Labeled Monoclonal Anti-FMC63 Antibody, Rabbit IgG (M4H08) (Cat. No. FM3-ABFMY2) and subsequently analyzed using FCS Express 7 software. Following the separation process, the percentage of Anti-CD19 CAR-T cells was enriched from an initial 37.29% to a final purity of 96.37%, indicating a highly successful isolation.

The Isolation of Anti-BCMA CAR-T Cells with Biotinylated Anti-G4S Linker Antibody and SA Nanobeads. Each 1E7 Anti-BCMA CAR-T cells were isolated with 0.6 µg Biotinylated Monoclonal Anti-G4S linker Antibody, Rabbit IgG (016), premium grade (Cat. No. G4S-BLM25G0) and appropriate SA Nanobeads. Both the isolated cells and non-isolated cells were stained with PE-labeled human BCMA/TNFRSF17 protein, His Tag, Star Staining (Cat. No. BCA-HP2H7) and subsequently analyzed using FCS Express 7 software. Following the separation process, the percentage of Anti-BCMA CAR-T cells was enriched from an initial 32.99% to a final purity of 99.01%, indicating a highly successful isolation.

The Isolation of Anti-CD19 CAR 293 Cells with Biotinylated Anti-G4S Linker Antibody and SA Nanobeads. Each 1E7 Anti-CD19 CAR 293 Cells were isolated with 0.2 µg Biotinylated Monoclonal Anti-G4S linker Antibody, Rabbit IgG (016), premium grade (Cat. No. G4S-BLM25G0) and appropriate SA Nanobeads. Both the isolated cells and non-isolated cells were stained with PE-Labeled Monoclonal Anti-FMC63 Antibody, Rabbit IgG (1G10) (0.03% Proclin) (Cat. No. FM3-PFM721) and subsequently analyzed using FCS Express 7 software. Following the separation process, the percentage of Anti-CD19 CAR 293 cells was enriched from an initial 22.69% to a final purity of 96.01%, indicating a highly successful isolation.

The Isolation of Anti-CD19 CAR 293 Cells with Biotinylated Anti-G4S Linker Antibody and SA Nanobeads. Each 1E7 Anti-CD19 CAR 293 Cells were isolated with 0.2 µg Biotinylated Monoclonal Anti-G4S linker Antibody, Rabbit IgG (016), premium grade (Cat. No. G4S-BLM25G0) and appropriate SA Nanobeads (Acro or Company M). The isolated cells were stained with PE-Labeled Monoclonal Anti-FMC63 Antibody, Rabbit IgG (1G10) (0.03% Proclin) (Cat. No. FM3-PFM721) and subsequently analyzed using FCS Express 7 software. The results show that using Biotinylated Monoclonal Anti-G4S linker Antibody, Rabbit IgG (016), premium grade (Cat. No. G4S-BLM25G0) combine with different SA nanobeads can all reach a final purity of over 95%, indicating a highly successful isolation.

Direct comparison of ACRO's three biotinylated anti-G4S products binding to Anti-MSLN CAR-293 cells. The overlapping MFI curves confirm identical functional activity across G4S-BLM25G0 (rHSA-containing, premium grade), G4S-BLM25A1 (CF, premium grade), and GMP-MG4S25 (CF, GMP).