製品詳細
Source
Monoclonal Anti-HA-H1N1(Influenza A/Sydney/5/2021) Antibody, Human IgG1 (1C10) is a recombinant chimeric monoclonal antibody expressed in HEK293, which combines the variable region of a mouse monoclonal antibody with human constant domain.
Antibody Type
Recombinant Monoclonal
Clone
1C10
Isotype
Human IgG1, Kappa
Host Species
Mouse
Specificity
This product is a specific antibody specifically reacts with HA.
Application
ApplicationRecommended UsageWestern Blot1-10 ug/mLELISA0.2-2 μg/mLCross Verification
This product can cross in Elisa with
Influenza A [Victoria/4897/2022] Hemagglutinin (HA) Protein, His Tag (Cat. No. HA1-V52H8).
Influenza A [Wisconsin/67/2022] Hemagglutinin (HA) Protein, His Tag (Cat. No. HA1-V52H7).
Influenza A (A/Georgia/12/2022) Hemagglutinin (HA) Protein, His Tag (Cat. No. HAE-V52H7).
This product No cross-reactivity in ELISA with Influenza A [A/Victoria/2570/2019] Hemagglutinin (HA) Protein, His Tag (Cat. No. HA1-V52H6).
Influenza A [A/Darwin/6/2021 (H3N2)] HA Protein, His Tag (Cat. No. HA2-V52H5).
Influenza A [A/Darwin/6/2021 (H3N2)] HA Protein, His Tag (Cat. No. HA2-V52H5).
Influenza A [A/Darwin/6/2021 (H3N2)] HA Protein, His Tag (Cat. No. HA2-V52H5).
Influenza A (Vietnam/1194/2004(H5N1)) Hemagglutinin (HA) Protein, His Tag (Cat. No. HA1-V52H9).
Influenza A (Guangdong/18SF020(H5N6)) Hemagglutinin (HA) Protein, His Tag (Cat. No.HA6-V52H3).
Influenza A (turkey/Germany-MV/R2472/2014(H5N8)) HA Protein, His Tag (Cat. No. HA8-V52H3).
Influenza A (A/Shanghai/02/2013(H7N9)) Hemagglutinin (HA) Protein, His Tag (Cat. No. HA9-V52H3).
Influenza A [A/guinea fowl/Hong Kong/WF10/99(H9N2)] Hemagglutinin (HA) Protein, His Tag (Cat. No. HA2-V52H7).
Influenza B [Austria/1359417/2021 (B/Victoria lineage)] Hemagglutinin (HA) Protein, His Tag (Cat. No. HAE-V52H3).
Influenza B [Phuket/3073/2013 (B/Yamagata lineage)] HA Protein, His Tag (Cat. No. HAE-V52H4).
Influenza A [A/Bangkok/1/1979 (H3N2)] HA, His Tag (Cat. No. HA2-V52H3).
Influenza A [A/Wisconsin/588/2019 (H1N1)] HA, His Tag (Cat. No. HA1-V52H3).
Influenza A Virus HA (H3N2) Protein, His Tag (Cat. No. H32-V52H3).
Influenza B (B/Singapore/INFTT-16-0610/2016) Hemagglutinin (HA) Protein, His Tag (Cat. No. HAE-V52H5).
Influenza B (B/Singapore/WUH4618/2021) Hemagglutinin (HA) Protein, His Tag (Cat. No. HAE-V52H6).
Influenza A (A/Sydney/1304/2022) Hemagglutinin (HA) Protein, His Tag (Cat. No. HA2-V52H9).
Influenza A Virus (A/District of Columbia/27/2023) HA (H3N2) Protein, His Tag (Cat. No. H32-V52H5).
Influenza A Virus (A/Croatia/10136RV/2023) HA (H3N2) Protein, His Tag (Cat. No. H32-V52H4).Purification
Protein A purified / Protein G purified.
Aggregation
Less than 10%, as determined by SEC-MALS.
Concentration
Please refer to the Certificate of Analysis (CoA).
Form
Lyophilized
Formulation
Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4), with trehalose as protectant.
Please contact us for customized product forms or formulations.
Reconstitution
Please refer to the Certificate of Analysis (CoA) for specific instructions.
Shipping
Lyophilized product is shipped at ambient temperature.
Storage
For long term storage, the product should be stored in a lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Notices
Product Specific Notices: For research use only.
ACRO Quality Management System
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データ表示
Bioactivity-ELISA

Immobilized Influenza A [Sydney/5/2021 (H1N1)] HA Protein, His Tag (Cat. No. HA1-V52H4) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-HA-H1N1(Influenza A/Sydney/5/2021) Antibody, Human IgG1 (1C10) (Cat. No. HA1-M757) with a linear range of 0.05-2 ng/mL (QC tested).
Protocol
Bioactivity-SPR

Monoclonal Anti-HA-H1N1 (Influenza A/Sydney/5/2021) Antibody, Human IgG1 (1C10) (Cat. No. HA1-M757) captured on Protein A Chip can bind Influenza A [Sydney/5/2021 (H1N1)] HA Protein, His Tag (Cat. No. HA1-V52H4) with an affinity constant of 0.334 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Western Blot

Western blot analysis of three recombinant Influenza A HA Proteins (all with His Tag): Influenza A [Victoria/4897/2022] HA Protein, His Tag (Cat. No. HA1-V52H8), Influenza A [Wisconsin/67/2022] HA Protein, His Tag (Cat. No. HA1-V52H7) and Influenza A [Sydney/5/2021 (H1N1)] HA Protein, His Tag (Cat. No. HA1-V52H4). Each protein was loaded at 200 ng per lane and detected using Monoclonal Anti-Hemagglutinin/HA (Influenza Virus) (A/Sydney/5/2021 (H1N1)) Antibody, Human IgG1 (1C10) at a 1:1000 dilution, followed by HRP-conjugated Goat Anti-Human IgG, Fcγ fragment specific secondary antibody at a 1:2000 dilution. Specific bands were detected at approximately 95 kDa, 100 kDa and 100 kDa for the three proteins.
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