Recombinant Monoclonal Anti-PD-1 Antibody, Mouse (2F3)

製品詳細

Background
Recombinant Monoclonal Anti-PD-1 Antibody, Mouse (2F3) is a PD-1 primary antibody for IHC application. Programmed cell death protein 1 (PDCD1) is an immune-inhibitory receptor expressed in activated T cells; it is involved in the regulation of T-cell functions, including those of effector CD8+ T cells. In addition, this protein can also promote the differentiation of CD4+ T cells into T regulatory cells. PDCD1 is expressed in many types of tumors including melanomas, and has demonstrated to play a role in anti-tumor immunity. Moreover, this protein has been shown to be involved in safeguarding against autoimmunity, however, it can also contribute to the inhibition of effective anti-tumor and anti-microbial immunity.

Host Species	Mouse
Isotype	Mouse IgG
Clone	2F3
Application	IHC-P
Dilution Ratio	1: 200 - 1: 1000
Form	Liquid
Clonality	Monoclonal
Reactivity	Human
Synonyms	PD1, CD279, PD-1, PDCD1
Concentration	0.9~1.1 mg/ml
Specificity	React with PD-1 protein on the membrane of target cell/tissue
Purification Tech	Protein A
Preservation	Tris buffer, 1% BSA

Experiment Protocol
LEICA Experiment Protocol
Storage
Shipped at 4°C, Blue Ice. Store at +4°C for short term (1-2 weeks). Store at -20°C for long term.
Avoid freeze / thaw cycle. Upon delivery aliquot.
ACRO Quality Management System

Sample Staining Data
Immunohistochemical analysis of paraffin embedded human cancer tissue labelled with HGS-S238 at 1:500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.