PE-Labeled Anti-SARS-CoV-2 Spike RBD Neutralizing Antibody, Chimeric mAb, Human IgG1 (AM122) (Site-specific conjugation)

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SPD-PM226-25tests
$380.00
In Stock - Delivery in 2-3 business days
SPD-PM226-200tests
$2345.00
In Stock - Delivery in 2-3 business days
総アイテム数 製品金額$ 0

製品詳細

  • Source

    PE-Labeled Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 (AM122) is a PE-labeled chimeric monoclonal antibody recombinantly expressed in HEK293 cells, which combines the variable regions of a mouse monoclonal antibody with human IgG1 constant domains.

  • Antibody Type

    Recombinant Monoclonal

  • Clone

    AM122

  • Isotype

    Human IgG1, Kappa

  • Host Species

    Mouse

  • Immunogen

    Recombinant SARS-CoV-2 Spike S1 protein

  • Conjugate

    PE

    Excitation Wavelength: 488 nm / 561 nm

    Emission Wavelength: 575 nm

  • Specificity

    This product is a specific antibody against SARS-CoV-2 Spike protein RBD domain. No cross-reactivity is detected with Spike protein RBD domain of other coronaviruses, including SARS-CoV, MERS-CoV, HCoV-229E, HCoV-NL63, HCoV-OC43 and HCoV-HKU1.

  • Application

    Flow Cytometry (Neutralizing assay).

  • Recommended Dilution

    1:50

  • Form

    Lyophilized

  • Formulation

    Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4), with trehalose and BSA as protectant.

    Please contact us for customized product forms or formulations.

  • Reconstitution

    Please refer to the Certificate of Analysis (CoA) for specific instructions.

  • Shipping

    Lyophilized product is shipped at ambient temperature.

  • Storage

    For long term storage, the product should be stored in a lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 24 months in lyophilized state;
    2. -70°C for 12 months under sterile conditions after reconstitution.
  • Notices

    Product Specific Notices: For research use only.

  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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データ表示

  • Bioactivity-FACS

     Spike RBD FACS

    5e5 of HEK293/SARS-CoV-2, Spike Stable Cell were stained with 100 μL of 1:50 dilution (2 μL stock solution in 100 μL FACS buffer) of PE-Labeled Anti-SARS-CoV-2 Spike RBD Neutralizing Antibody, Chimeric mAb, Human IgG1 (AM122) (Cat. No. SPD-PM226) and Isotype control antibody respectively. PE signal was used to evaluate the binding activity (QC tested).

    Protocol

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It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

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