Monoclonal Anti-Rotavirus A VP4 Antibody, Human IgG1 (4E1)

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VP4-M765-100ug
$415.00
In Stock - Delivery in 2-3 business days
VP4-M765-1mg
$2735.00
In Stock - Delivery in 2 weeks
総アイテム数 製品金額$ 0

製品詳細

  • Source

    Chimeric Monoclonal Anti-Rotavirus A VP4 Antibody, Human IgG1 (4E1) is recombinantly expressed in HEK293. It consists of mouse variable regions genetically fused to human IgG1 constant domains.

  • Antibody Type

    Recombinant Monoclonal

  • Clone

    4E1

  • Isotype

    Human IgG1, Kappa

  • Host Species

    Mouse

  • Immunogen

    Recombinant Rotavirus A (strain RVA/Human/United States/Wa/1974/G1P1A[8]) VP4 Protein is expressed from human 293 cells

  • Specificity

    Specifically recognizes Rotavirus A VP4.

  • Application

    Application
    Recommended Use / Performance
    ELISA
    0.2-2 μg/mL
  • Purification

    Protein A purified.

  • Aggregation

    Less than 10%, as determined by SEC-MALS.

  • Concentration

    Please refer to the Certificate of Analysis (CoA).

  • Form

    Lyophilized

  • Formulation

    Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4), with trehalose as protectant.

    Please contact us for customized product forms or formulations.

  • Reconstitution

    Please refer to the Certificate of Analysis (CoA) for specific instructions.

  • Shipping

    Lyophilized product is shipped at ambient temperature.

  • Storage

    For long term storage, the product should be stored in a lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • Notices

    Product Specific Notices: For research use only.

  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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データ表示

  • Bioactivity-ELISA

     VP4 (Rotavirus A) ELISA

    Immobilized Rotavirus A VP4 Protein, His Tag (Cat. No. VP4-R5243) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Rotavirus A VP4 Antibody, Human IgG1 (4E1) (Cat. No. VP4-M765) with a linear range of 0.03-2 ng/mL (QC tested).

    Protocol
  • Bioactivity-SPR

     VP4 (Rotavirus A) SPR

    Monoclonal Anti-Rotavirus A VP4 Antibody, Human IgG1 (4E1) (Cat. No. VP4-M765) captured on Protein A Chip can bind Rotavirus A VP4 Protein, His Tag (Cat. No. VP4-R5243) with an affinity constant of 1.25 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).

    Protocol

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よくある質問

  • product

    Can lyophilized proteins remain stable during room-temperature shipping or temporary room-temperature exposure?

    In general, lyophilized proteins remain stable during room-temperature shipping or temporary exposure to room-temperature conditions.


    Many ACROBiosystems recombinant proteins are supplied in lyophilized (freeze-dried) form. The lyophilization process effectively removes moisture from the product, helping reduce degradation-related reactions and improve protein stability during transportation and storage.


    To evaluate the stability of lyophilized proteins under normal temperature conditions, ACROBiosystems conducted a stability study on 11 representative lyophilized protein products at 37°C. The study demonstrated that these proteins maintained good quality stability after storage at 37°C for approximately 20 days. Based on these validation results, temporary room-temperature exposure during shipping or handling is generally not expected to have a significant impact on product quality or downstream application performance.


    To ensure optimal long-term stability, products should be stored according to the storage conditions specified in the product Certificate of Analysis (COA) upon receipt.


    Supporting Document


バックグランド

Rotavirus is the leading cause of severe, watery diarrhea in infants and children less than 5 years old. It is estimated that around three-quarters of infants and children had rotavirus diarrhea before the age of 12 months and over millions of them were hospitalized due to the rotavirus infection. The rotavirus has various structural viral protein (VPs) and non-structural protein (NSPs), among them, the VP4 and VP7 together determines the serotypes of the virus, with the VP4 determines the P-type and VP-7 determines the G-type. The reassortment of these proteins leads to the diversity of the rotavirus strains, making the VP4 and VP7 the important target for vaccine studies.

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