Human Cadherin-11 Protein, His Tag (MALS verified)

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CA1-H52H3-100ug
$395.00
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CA1-H52H3-1mg (500ug X 2)
$2,615.00
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CA1-H52H3
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Synonyms

Cadherin-11

Source

Human Cadherin-11 Protein, His Tag (CA1-H52H3) is expressed from human 293 cells (HEK293). It contains AA Gly 54 - Thr 617 (Accession # P55287-1).

Predicted N-terminus: Gly 54

Molecular Characterization

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 63.8 kDa. The protein migrates as 65-80 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin

Less than 1.0 EU per μg by the LAL method / rFC method.

Purity

>90% as determined by SDS-PAGE.

>95% as determined by SEC-MALS.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, 0.5 M Arginine, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Shipping and Storage

This product is shipped at ambient temperature.

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:
  • -20°C to -70°C for 12 months in lyophilized state;
  • -70°C for 3 months under sterile conditions after reconstitution.

バックグランド

Cadherin-11 is a type II cadherin which mediates cell-to-cell homophilic interactions. Cadherin-11 is mainly expressed on mesenchymal cells and is essential for tissue migration and organization during embryogenesis, while its expression on epithelial cancer cells is implicated in tumor progression and metastasis suggesting a potential role in the epithelial-to-mesenchymal transition phenomenon. Expression of this particular cadherin in osteoblastic cell lines, and its upregulation during differentiation, suggests a specific function in bone development and maintenance. In addition, cadherin-11 has been shown to increase the invasive capacity of Fibroblast-like Synoviocytes (FLS) and consequently be involved in the formation of the rheumatoid pannus.