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製品詳細
Synonyms
CAIX, CA9, CA-IX, G250, MN, P54, 58N, pMW1
Source
Human Carbonic Anhydrase IX (138-414), His Tag (CA9-H5220) is expressed from human 293 cells (HEK293). It contains AA Gln 138 - Asp 414 (Accession # NP_001207.2).
Predicted N-terminus: Gln 138
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 30.9 kDa. The protein migrates as 34 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
The protein is designed as a dimer.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Supplied as 0.2 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Shipping and Storage
This product is supplied and shipped on dry ice.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- The product MUST be stored at -70°C or lower upon receipt;
- -70°C for 3 months under sterile conditions.
ACRO Quality Management System
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データ表示
SDS-PAGE
Human Carbonic Anhydrase IX (138-414), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Human Carbonic Anhydrase IX (138-414), His Tag (Cat. No. CA9-H5220) is more than 95% and the molecular weight of this protein is around 60-70 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human Carbonic Anhydrase IX (138-414), His Tag (Cat. No. CA9-H5220) at 1 μg/mL (100 μL/well) can bind Anti-Carbonic Anhydrase IX Antibody, Human IgG1 (Cat. No. AM213) with a linear range of 0.3-1.2 ng/mL (QC tested).
Protocol
Bioactivity-SPR
Anti-Carbonic Anhydrase IX Antibody captured on Protein A Chip can bind Human Carbonic Anhydrase IX (138-414), His Tag (Cat. No. CA9-H5220) with an affinity constant of 1.29 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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バックグランド
Carbonic anhydrase 9 (CA9 / CAIX) is also known as Membrane antigen MN (MN), Renal cell carcinoma-associated antigen G250, which belongs to the alpha-carbonic anhydrase family. CA9 / CAIX with an optimal activity at pH 6.49. Reversible hydration of carbon dioxide. CA IX participates in pH regulation. CA9 may be involved in the control of cell proliferation and transformation. CA-IX appears to be a novel specific biomarker for a cervical neoplasia.
最新の進展
医薬品開発の進捗- 英語名：
Carbonic anhydrase IX
- カテゴリ：
- 市販の医薬品数：
0 詳細
- 治験医薬品数：
16 詳細
- 最新の開発ステージ：
Phase 3 Clinical
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