Bst DNA Polymerase

Product Details

Bst DNA Polymerase is a recombinant form of Bacillus stearothermophilus DNA Polymerase, Large Fragment produced in Escherichia coli. Bst DNA Polymerase contains 5´→3´ DNA polymerase activity with either DNA or RNA templates and strong strand displacement activity, but lacks 5´→3´ and 3´→5´ exonuclease activity.

Materials Provided

Application

Isothermal amplification (LAMP, RT-LAMP).
DNA sequencing of rich GC sequence.
Rapid sequencing of DNA templates (ng).

Unit Definition

One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 65°C.

Purity

>90% as determined by SDS-PAGE.

Enzyme Activity

>8 U/μL

Formulation

Supplied as 0.2 μm filtered solution in 10 mM Tris-HCl, pH7.1 with glycerol as protectant.

Contact us for customized product form or formulation.

Shipping and Storage

This product is supplied and shipped on dry ice.

This product is stable after storage at:

1. The product MUST be stored at -20°C or lower upon receipt.
2. -20°C for 3 months under sterile conditions.