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製品詳細
Synonyms
DNAM1, CD226, PTA1
Source
Human DNAM-1, Fc Tag (DN1-H5257) is expressed from human 293 cells (HEK293). It contains AA Glu 19 - Asn 247 (Accession # NP_006557).
Predicted N-terminus: Glu 19
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 52.6 kDa. The protein migrates as 65-85 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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データ表示
SDS-PAGE
Human DNAM-1, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human DNAM-1, Fc Tag (Cat. No. DN1-H5257) is more than 95% and the molecular weight of this protein is around 125-155 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Rhesus macaque CD155 (Cat. No. CD5-R52H5) at 5 μg/mL (100 μL/well) can bind Human DNAM-1, Fc Tag (Cat. No. DN1-H5257) with a linear range of 2-31 ng/mL (QC tested).
Protocol
Bioactivity-BLI
Loaded Human DNAM-1, Fc Tag (Cat. No. DN1-H5257) on Protein A Biosensor, can bind Human CD155, His Tag (Cat. No. CD5-H5223) with an affinity constant of 1.1 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Loaded Human DNAM-1, Fc Tag (Cat. No. DN1-H5257) on Protein A Biosensor, can bind Human Nectin-2, His Tag (Cat. No. PV2-H52E2) with an affinity constant of 0.66 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Bioactivity-FACS
FACS assay shows that recombinant Human DNAM-1 Protein, Fc Tag (Cat. No. DN1-H5257) can bind to 293T cell overexpressing human CD155. The concentration of DNAM-1 used is 0.03 μg/ml (Routinely tested).
Protocol
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最新の進展
医薬品開発の進捗- 英語名：
CD226 antigen
- カテゴリ：
- 市販の医薬品数：
0 詳細
- 治験医薬品数：
0 詳細
- 最新の開発ステージ：
Preclinical
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