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製品詳細
Synonyms
FCER1A, FCE1A, FcERI
Source
Human Fc epsilon RI alpha, Fc Tag (FCA-H5259) is expressed from human 293 cells (HEK293). It contains AA Val 26 - Leu 204 (Accession # P12319-1).
Predicted N-terminus: Val 26
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 47.3 kDa. The protein migrates as 60-75 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 12 months under sterile conditions after reconstitution.
ACRO Quality Management System
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データ表示
SDS-PAGE
Human Fc epsilon RI alpha, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human Fc epsilon RI alpha, Fc Tag (Cat. No. FCA-H5259) is more than 95% and the molecular weight of this protein is around 125-135 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human Fc epsilon RI alpha, Fc Tag (Cat. No. FCA-H5259) at 5 μg/mL (100 μL/well) can bind Human IgE Fc, His Tag (Cat. No. IGE-H52H9) with a linear range of 0.6-20 ng/mL (Routinely tested).
Protocol
Bioactivity-SPR
Human Fc epsilon RI alpha, Fc Tag (Cat. No. FCA-H5259) immobilized on CM5 Chip can bind Human IgE Fc, His Tag (Cat. No. IGE-H52H9) with an affinity constant of 0.585 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Bioactivity-BLI
Loaded Human Fc epsilon RI alpha, Fc Tag (Cat. No. FCA-H5259) on AHC Biosensor, can bind Immunoglobulin E, Human Plasma with an affinity constant of 0.696 nM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).
Protocol
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最新の進展
医薬品開発の進捗- 英語名：
IgE Fc receptor, alpha-subunit
- カテゴリ：
- 市販の医薬品数：
1 詳細
- 治験医薬品数：
1 詳細
- 最新の開発ステージ：
Approved
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