Human FcRn / FCGRT&B2M Heterodimer Protein, Strep Tag&Tag Free (MALS & SPR verified)

Synonyms

FcRn, FCGRT & B2M

Source

Human FCGRT&B2M Heterodimer Protein, Strep Tag&Tag Free (FCM-H5283) is expressed from human 293 cells (HEK293). It contains AA Ala 24 - Ser 297 (FCGRT) & Ile 21 - Met 119 (B2M) (Accession # P55899-1 (FCGRT) & P61769-1 (B2M)).

Predicted N-terminus: Ala 24 (FCGRT) & Ile 21 (B2M)

Molecular Characterization

Other Tags and Version Biotin & Other Labeled Version

Human FCGRT&B2M Heterodimer Protein, Strep Tag&Tag Free, produced by co-expression of FCGRT and B2M, has a calculated MW of 33.8 kDa (FCGRT) and 11.7 kDa (B2M). Subunit FCGRT is fused with Strep II-tag at the C-terminus and subunit Beta-2 microglobulin (B2M) contains no tag. The reducing (R) protein migrates as 40-43 kDa (FCGRT) and 12 kDa (B2M) when calibrated against Star Ribbon Pre-stained Protein Marker respectively due to glycosylation.

Endotoxin

Less than 1.0 EU per μg by the LAL method / rFC method.

Purity

>90% as determined by SDS-PAGE.

>95% as determined by SEC-MALS.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Shipping and Storage

This product is shipped at ambient temperature.

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;
2. -70°C for 3 months under sterile conditions after reconstitution.

ACRO Quality Management System

1. QMS(ISO, GMP)
2. Quality Advantages
3. Quality Control Process