Biotinylated Rhesus macaque Glypican 2 / GPC2 Protein, His,Avitag™ (MALS verified)

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Cat. No. / Size
Price
Qty
GP2-R82E3-25ug
$350.00
GP2-R82E3-200ug
$1380.00
ETA of in-stock products:2 business days

Product Details

  • Synonyms

    Glypican 2, GPC2

  • Source

    Biotinylated Rhesus macaque Glypican 2 Protein, His,Avitag (GP2-R82E3) is expressed from human 293 cells (HEK293). It contains AA Ser 24 - Ser 552 (Accession # XP_001103286).

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  • Molecular Characterization

    Glypican 2 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 61.6 kDa. The protein migrates as 30-33 kDa and 35-38 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).

  • Labeling

    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

  • Protein Ratio

    Passed as determined by the HABA assay / binding ELISA.

  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    Glypican 2 SDS-PAGE

    Biotinylated Rhesus macaque Glypican 2 Protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    Glypican 2 SEC-MALS

    The purity of Biotinylated Rhesus macaque Glypican 2 Protein, His,Avitag (Cat. No. GP2-R82E3) is more than 85% and the molecular weight of this protein is around 50-80 kDa verified by SEC-MALS.

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  • Bioactivity-ELISA

     Glypican 2 ELISA

    Immobilized Human Midkine Protein, premium grade (Cat. No. MIE-H5117) at 5 μg/mL (100 μL/well) can bind Biotinylated Rhesus macaque Glypican 2 Protein, His,Avitag (Cat. No. GP2-R82E3) with a linear range of 0.001-0.5 μg/mL (QC tested).

    Protocol

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Background

Glypican 2 (GPC2), also known cerebroglycan, is a glycophosphatidylinositol-linked integral membrane heparan sulfate proteoglycan found in the developing nervous system. Cerebroglycan participates in cell adhesion and is thought to regulate the growth and guidance of axons. Cerebroglycan has especially high affinity for laminin-1. GPC2 silencing inactivates Wnt/β-catenin signaling and reduces the expression of N-Myc, an oncogenic driver of neuroblastoma tumorigenesis. Immunotoxins and chimeric antigen receptor (CAR) T cells targeting GPC2 inhibit neuroblastoma growth in mouse models. A GPC3 specific antibody drug conjugate (ADC) can also inhibit neuroblastoma cell proliferation.

Recent Advances

 
Drug Development Progress
  • English Name:

    Glypican-2

  • Category:

  • Approved Drugs:

    0 Details

  • Drugs in Clinical Trials:

    0 Details

  • Highest Development Stage:

    Phase 1 Clinical

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