Biotinylated Human HGF R / c-MET Protein, His,Avitag™

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製品番号/仕様
価格
数量
MET-H82E3-25ug
$405.00
MET-H82E3-200ug
$1620.00
ETA of in-stock products:2 business days
総アイテム数 製品金額$ 0

製品詳細

  • Synonyms

    MET, AUTS9, HGFR, RCCP2, c-Met

  • Source

    Biotinylated Human HGF R Protein, His,Avitag (MET-H82E3) is expressed from human 293 cells (HEK293). It contains AA Glu 25 - Asp 741 (Accession # P08581-1).

    Request for sequence
  • Molecular Characterization

    HGF R Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 84.4 kDa. The protein migrates as 60-70 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).

  • Labeling

    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

  • Protein Ratio

    Passed as determined by the HABA assay / binding ELISA.

  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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データ表示

  • SDS-PAGE

    HGF R SDS-PAGE

    Biotinylated Human HGF R Protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

  • Bioactivity-ELISA

     HGF R ELISA

    Immobilized Biotinylated Human HGF R Protein, His,Avitag (Cat. No. MET-H82E3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human HGF, Fc Tag (Cat. No. HGF-H5253) with a linear range of 0.2-2 ng/mL (QC tested).

    Protocol
  •  HGF R ELISA

    Immobilized Human HGF, Fc Tag (Cat. No. HGF-H5253) at 2 μg/mL (100 μL/well) can bind Biotinylated Human HGF R Protein, His,Avitag (Cat. No. MET-H82E3) with a linear range of 1-31 ng/mL (Routinely tested).

    Protocol

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バックグランド

This gene encodes a member of the receptor tyrosine kinase family of proteins and the product of the proto-oncogene MET. The encoded preproprotein is proteolytically processed to generate alpha and beta subunits that are linked via disulfide bonds to form the mature receptor. Further processing of the beta subunit results in the formation of the M10 peptide, which has been shown to reduce lung fibrosis. Binding of its ligand, hepatocyte growth factor, induces dimerization and activation of the receptor, which plays a role in cellular survival, embryogenesis, and cellular migration and invasion. Mutations in this gene are associated with papillary renal cell carcinoma, hepatocellular carcinoma, and various head and neck cancers. Amplification and overexpression of this gene are also associated with multiple human cancers

最新の進展

 
医薬品開発の進捗
  • 英語名:

    Hepatocyte growth factor receptor

  • カテゴリ:

  • 市販の医薬品数:

    13 詳細

  • 治験医薬品数:

    74 詳細

  • 最新の開発ステージ:

    Approved

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