APC-Labeled Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQCFL) Tetramer Protein

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製品番号/仕様
価格
数量
HL1-HA2H4-25ug
$410.00
HL1-HA2H4-200ug (100ug X 2)
$2350.00
ETA of in-stock products:2 business days
総アイテム数 製品金額$ 0

製品詳細

  • Synonyms

    HLA-A*0201 & B2M & NY-ESO-1 (SLLMWITQCFL)

  • Source

    APC-Labeled Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQCFL) Tetramer Protein (HL1-HA2H4) is expressed from human 293 cells (HEK293). It contains AA Gly 25 - Ile 308 (HLA-A*02:01) & Ile 21 - Met 119 (B2M) & SLLMWITQCFL peptide (Accession # AAA59606.1 (HLA-A*02:01) & P61769-1 (B2M) & SLLMWITQCFL).

    Predicted N-terminus: Gly 25 & Ile 21

    Request for sequence
  • Molecular Characterization

    APC-Labeled Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQCFL) Tetramer Protein is assembled by biotinylated monomer and APC-labeled streptavidin.

    Biotinylated Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQCFL) Complex Protein is produced by co-expression of HLA and B2M loaded with NY-ESO-1 peptide. Biotinylated Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQCFL) Complex Protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

  • Conjugate

    APC

    Excitation Wavelength: 640 nm

    Emission Wavelength: 661 nm

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, 1% BSA, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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  • Bioactivity-ELISA

     HLA-A*0201 & B2M & NY-ESO-1 (SLLMWITQCFL) ELISA

    Immobilized APC-Labeled Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQCFL) Tetramer Protein (Cat. No. HL1-HA2H4) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-HLA class I Antibody, Human IgG1 (W632) with a linear range of 0.1-3 ng/mL (QC tested).

    Protocol
  •  HLA-A*0201 & B2M & NY-ESO-1 (SLLMWITQCFL) ELISA

    Immobilized APC-Labeled Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQCFL) Tetramer Protein (Cat. No. HL1-HA2H4) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-B2M Antibody, Human IgG1 with a linear range of 0.1-3 ng/mL (Routinely tested).

    Protocol

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バックグランド

NY-ESO-1, which is also well-known as New York esophageal squamous cell carcinoma 1, is an efficient target for cancer immunotherapy. This antigen is a member of cancer-testis antigens (CTAs) and is highly expressed in various cancers, including melanoma, ovarian, cervical cancer, etc. Adoptive T cell therapy with HLA-A2 restricted NY-ESO-1 transduced CD8+ T cells has improved the clinical response rates and overall survival of treatment-refractory melanoma patients. The Human HLA-A*0201 NY-ESO-1 (SLLMWITQCFL) complex protein is a complex of HLA-A*0201 of the MHC Class I, B2M and SLLMWITQCFL peptide of the NY-ESO-1.

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