Biotinylated Human HLA-A*24:02&B2M&NY-ESO-1 (LLMWITQCF) Complex Protein (Monomer, MALS verified)

Synonyms

HLA-A*2402 & B2M & NY-ESO-1 (LLMWITQCF)

Source

Biotinylated Human HLA-A*24:02&B2M&NY-ESO-1 (LLMWITQCF) Complex Protein (HL1-H82E3) is expressed from human 293 cells (HEK293). It contains AA Gly 25 - Thr 305 (HLA-A*24:02) & Ile 21 - Met 119 (B2M) & LLMWITQCF peptide (Accession # AAA59600.1 (HLA-A*24:02) & P61769 (B2M) & LLMWITQCF).

Predicted N-terminus: Gly 25 & Ile 21

Molecular Characterization

Biotinylated Human HLA-A*24:02&B2M&NY-ESO-1 (LLMWITQCF) Complex Protein is produced by co-expression of HLA and B2M loaded with NY-ESO-1 peptide.

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

The protein has a calculated MW of 35.8 kDa and 11.7 kDa. The protein migrates as 38-42 kDa and <14 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Labeling

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Purity

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

Formulation

Supplied as 0.2 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

Shipping and Storage

This product is supplied and shipped on dry ice.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. The product MUST be stored at -70°C or lower upon receipt;
2. -70°C for 3 months under sterile conditions.

ACRO Quality Management System

1. QMS(ISO, GMP)
2. Quality Advantages
3. Quality Control Process