Human HLA-G protein, His Tag

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製品番号/仕様
価格
数量
HLG-H51H3-100ug (50ug X 2)
$380.00
HLG-H51H3-1mg (50ug X 20)
$2650.00
ETA of in-stock products:2 business days
総アイテム数 製品金額$ 0

製品詳細

  • Synonyms

    HLA-G, HLA class I histocompatibility antigen, alpha chain G, HLAG, HLA-6.0

  • Source

    Human HLA-G protein, His Tag (HLG-H51H3) is expressed from E. coli cells. It contains AA Gly 25 - Ala 206 (Accession # P17693-1).

    Predicted N-terminus: Met

    Request for sequence
  • Molecular Characterization

    HLA-G Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 23 kDa. The protein migrates as 23-26 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).

  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, 0.5 M Arginine, 0.5 M NaCl, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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データ表示

  • SDS-PAGE

    HLA-G SDS-PAGE

    Human HLA-G protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

  • Bioactivity-ELISA

     HLA-G ELISA

    Immobilized Human HLA-G protein, His Tag (Cat. No. HLG-H51H3) at 5 μg/mL (100 μL/well) can bind Human KIR2DL4, Fc Tag (Cat. No. KI4-H5255) with a linear range of 1.6-50 ng/mL (QC tested).

    Protocol
  •  HLA-G ELISA

    Immobilized Human HLA-G protein, His Tag (Cat. No. HLG-H51H3) at 5 μg/mL (100 μL/well) can bind Human LILRB2, Fc Tag (Cat. No. LI2-H5253) with a linear range of 0.3-2 ng/mL (Routinely tested).

    Protocol

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バックグランド

Human leukocyte antigen-G (HLA-G) is a group of closely linked genes located on the short arm of human chromosome 6. Hla-g belongs to a non-classical major histocompatibility complex. MHC class I molecules are selectively highly expressed in extravvillous trophoblast cells invading the uterine decidual membrane.The gene structure of HLA-G is similar to that of HLA-A,HLA-B and HLA-C, but the termination code appears in advance so that the intracellular segment of protein product encoded by HLA-G is only 6 amino acids, which is significantly shorter than the 30 amino acids of classical HLA classI antigen. The Human HLA-G & B2M & RIIPRHLQL Complex Protein is a complex of HLA-G of the MHC Class I, B2M and RMFPNAPYL peptide.

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