Biotinylated Human IL-3 R alpha / CD123 Protein, Avitag™ (MALS verified)

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製品番号/仕様
価格
数量
ILA-H8214-25ug
$380.00
ILA-H8214-200ug
$1490.00
ETA of in-stock products:2 business days
総アイテム数 製品金額$ 0

製品詳細

  • Synonyms

    IL3R, IL3RA, IL-3Ra, IL-3R-alpha, IL3RAY, IL3RX, IL3RY, CD123 antigen, CD123, hIL3Ra, hIL-3Ra, MGC34174, IL-3 R alpha

  • Source

    Biotinylated Human IL-3 R alpha Protein, Avitag (ILA-H8214) is expressed from human 293 cells (HEK293). It contains AA Thr 19 - Arg 305 (Accession # P26951-1).

    Predicted N-terminus: Thr 19

    Request for sequence
  • Molecular Characterization

    IL-3 R alpha Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries an Avi tag (Avitag™) at the C-terminus.

    The protein has a calculated MW of 35.3 kDa. The protein migrates as 53-63 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling

    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

  • Protein Ratio

    Passed as determined by the HABA assay / binding ELISA.

  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Shipping and Storage

    This product is shipped at ambient temperature.

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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データ表示

  • SDS-PAGE

    IL-3 R alpha SDS-PAGE

    Biotinylated Human IL-3 R alpha Protein, Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

  • SEC-MALS

    IL-3 R alpha SEC-MALS

    The purity of Biotinylated Human IL-3 R alpha Protein, Avitag (Cat. No. ILA-H8214) is more than 85% and the molecular weight of this protein is around 43-68 kDa verified by SEC-MALS.

    Report
  • Bioactivity-ELISA

     IL-3 R alpha ELISA

    Immobilized Biotinylated Human IL-3 R alpha Protein, Avitag (Cat. No. ILA-H8214) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-IL-3 R alpha Antibody, Human IgG1 with a linear range of 0.1-2 ng/mL (QC tested).

    Protocol
  • Bioactivity-SPR

     IL-3 R alpha SPR

    Biotinylated Human IL-3 R alpha Protein, Avitag (Cat. No. ILA-H8214) immobilized on SA Chip can bind Human IL-3 Protein, His Tag (Cat. No. IL3-H52H9) with an affinity constant of 0.314 μM as determined in a SPR assay (Biacore 8K) (Routinely tested).

    Protocol

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バックグランド

Interleukin 3 receptor alpha (low affinity) (IL3RA), also known as CD123 (Cluster of Differentiation 123) is a 70-kD glycoprotein member of the hematopoietin receptor superfamily. This protein associates with a beta subunit common to the receptors for IL-5 and granulocyte-macrophage colony-stimulating factor (GM-CSF) to form a high-affinity receptor for IL-3. The interleukin-3 receptor α chain (CD123) has been identified as a potential immunotherapeutic target because it is overexpressed in AML compared with normal hematopoietic stem cells.

最新の進展

 
医薬品開発の進捗
  • 英語名:

    Interleukin-3 receptor subunit alpha

  • カテゴリ:

  • 市販の医薬品数:

    1 詳細

  • 治験医薬品数:

    40 詳細

  • 最新の開発ステージ:

    Approved

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