Cynomolgus Integrin alpha 5 beta 1 (ITGA5&ITGB1) Heterodimer Protein, His Tag&Tag Free (MALS verified)

Synonyms

Integrin alpha 5 beta 1, ITGA5&ITGB1

Source

Cynomolgus ITGA5&ITGB1 Heterodimer Protein, His Tag&Tag Free (IT1-C52W7) is expressed from human 293 cells (HEK293). It contains AA Phe 42 - Tyr 999 (ITGA5) & Gln 161 - Asp 868 (ITGB1) (Accession # XP_005571144.3 (ITGA5) & A0A7N9D0D7 (ITGB1)).

Predicted N-terminus: Phe 42 (ITGA5) & Gln 161 (ITGB1)

Molecular Characterization

Other Tags and Version Biotin & Other Labeled Version

Cynomolgus ITGA5&ITGB1 Heterodimer Protein, His Tag&Tag Free, produced by co-expression of ITGA5 and ITGB1, has a calculated MW of 111.2 kDa (ITGA5) & 83.6 kDa (ITGB1). Subunit ITGA5 is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB1 contains no tag but a basic tail at the C-terminus. The protein migrates as 130-160 kDa (ITGA5) and 95-110 kDa (ITGB1) when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (NR) condition (SDS-PAGE) due to glycosylation.

The protein is designed as a heterodimer.

Endotoxin

Less than 1.0 EU per μg by the LAL method / rFC method.

Purity

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

Formulation

Lyophilized from 0.22 μm filtered solution in 50 mM HEPES, 150 mM NaCl, pH7.5 with trehalose as protectant.

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Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;
2. -70°C for 3 months under sterile conditions after reconstitution.

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