製品詳細
Synonyms
integrin alpha-7 isoform X1B beta 1, ITGA7X1B&ITGB1, ITGA7X1BB1
Source
Cynomolgus ITGA7X1B&ITGB1 Heterodimer Protein, Tag Free&His Tag (IT1-C52W3) is expressed from human 293 cells (HEK293). It contains AA Phe 34 - Pro 1081 (ITGA7X1B) & Gln 21 - Asp 728 (ITGB1) (Accession # XP_005571182.1 (ITGA7X1B) & XP_015311227.1 (ITGB1)).
Predicted N-terminus: Phe 34 (ITGA7X1B) & Gln 21 (ITGB1)
Request for sequenceMolecular Characterization

Other Tags and Version Biotin & Other Labeled Version
Cynomolgus ITGA7X1B&ITGB1 Heterodimer Protein, Tag Free&His Tag, produced by co-expression of ITGA7X1B and ITGB1, has a calculated MW of 115.1 kDa (ITGA7X1B) and 85.0 kDa (ITGB1). Subunit ITGA7X1B contains no tag but an acidic tail at the C-terminus and subunit ITGB1 contains a polyhistidine tag and a basic tail at the C-terminus. The non-reducing (NR) protein migrates as 95-115 kDa due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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データ表示
SDS-PAGE

Cynomolgus ITGA7X1B&ITGB1 Heterodimer Protein, Tag Free&His Tag on SDS-PAGE under non-reducing (NR) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS

The purity of Cynomolgus ITGA7X1B&ITGB1 Heterodimer Protein, Tag Free&His Tag (Cat. No. IT1-C52W3) is more than 95% and the molecular weight of this protein is around 210-250 kDa verified by SEC-MALS.
Report
Bioactivity-SPR

Human Laminin 421 Protein, premium grade (Cat. No. LA4-H5218) immobilized on CM5 Chip can bind Cynomolgus ITGA7X1B&ITGB1 Heterodimer Protein, Tag Free&His Tag (Cat. No. IT1-C52W3) with an affinity constant of 24.5 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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