Biotinylated Human Langerin Protein, Avitag™,His Tag

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製品番号/仕様
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数量
LAN-H82Q3-20ug
$350.00
In Stock - Delivery in 2-3 business days
LAN-H82Q3-200ug
$1380.00
In Stock - Delivery in 2 weeks
総アイテム数 製品金額$ 0

製品詳細

  • Synonyms

    CLEC4K, CD207, Langerin

  • Source

    Biotinylated Human Langerin Protein, Avitag,His Tag (LAN-H82Q3) is expressed from human 293 cells (HEK293). It contains AA Pro 65 - Pro 328 (Accession # Q9UJ71.2).

    Predicted N-terminus: Gly

    Request for sequence
  • Molecular Characterization

    Langerin Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries an Avi tag (Avitag™) at the N-terminus, followed by a polyhistidine tag.

    The protein has a calculated MW of 33.4 kDa. The protein migrates as 38-40 kDa and 42-43 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling

    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

  • Protein Ratio

    Passed as determined by the SABA assay / HABA assay / binding ELISA.

  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Shipping and Storage

    This product is shipped at ambient temperature.

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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データ表示

  • SDS-PAGE

    Langerin SDS-PAGE

    Biotinylated Human Langerin Protein, Avitag,His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

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よくある質問

  • product

    Can lyophilized proteins remain stable during room-temperature shipping or temporary room-temperature exposure?

    In general, lyophilized proteins remain stable during room-temperature shipping or temporary exposure to room-temperature conditions.


    Many ACROBiosystems recombinant proteins are supplied in lyophilized (freeze-dried) form. The lyophilization process effectively removes moisture from the product, helping reduce degradation-related reactions and improve protein stability during transportation and storage.


    To evaluate the stability of lyophilized proteins under normal temperature conditions, ACROBiosystems conducted a stability study on 11 representative lyophilized protein products at 37°C. The study demonstrated that these proteins maintained good quality stability after storage at 37°C for approximately 20 days. Based on these validation results, temporary room-temperature exposure during shipping or handling is generally not expected to have a significant impact on product quality or downstream application performance.


    To ensure optimal long-term stability, products should be stored according to the storage conditions specified in the product Certificate of Analysis (COA) upon receipt.


    Supporting Document


バックグランド

Langerin (CD207) is a crucial C-type lectin receptor (CLR) primarily expressed by Langerhans cells (LCs) in the epidermis. It functions as a pattern recognition receptor (PRR), utilizing its extracellular domain to specifically bind carbohydrate structures on pathogens (e.g., mannose, fucose). Uniquely associated with Birbeck granules, Langerin mediates efficient antigen internalization and processing, thereby enabling LCs to initiate adaptive immune responses and restrict certain infections. Its distinct expression also serves as a key diagnostic marker for Langerhans Cell Histiocytosis (LCH).

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