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製品詳細
Synonyms
Latent TGF-beta 1, Latent TGFB1, TGFB1, CED, DPD1, LAP, TGF-beta-1, TGFB
Source
Cynomolgus Latent TGF-Beta 1 (C33S), His Tag (TG1-C5243) is expressed from human 293 cells (HEK293). It contains AA Leu 30 - Ser 390 (C33S) (Accession # XP_005589396.1).
Predicted N-terminus: His & Leu 280
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the N-terminus. The precursor proprotein is partially cleaved by FURIN to form mature Transforming growth factor beta-1 (TGF-beta-1) and Latency-associated peptide (LAP) chains with calculated MW of 12.7 kDa and 30.4 kDa respectively. The protein migrates as 50-60 kDa (precursor proprotein), 38-45 kDa (LAP) and 13 kDa (TGFB1) under reducing (R) condition (SDS-PAGE) due to glycosylation.
The protein is designed as a dimer.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
>90% as determined by SEC-HPLC.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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データ表示
SDS-PAGE
Cynomolgus Latent TGF-Beta 1 (C33S), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
SEC-HPLC
The purity of Cynomolgus Latent TGF-Beta 1 (C33S), His Tag (Cat. No. TG1-C5243) was greater than 90% as determined by SEC-HPLC.
Bioactivity-ELISA
Immobilized Cynomolgus Latent TGF-Beta 1 (C33S), His Tag (Cat. No. TG1-C5243) at 2 μg/mL (100 μL/well) can bind Biotinylated Human ITGAV&ITGB6 Heterodimer Protein, His,Avitag&Tag Free (Cat. No. IT6-H82E4) with a linear range of 1-31 ng/mL (QC tested).
Protocol
Immobilized Cynomolgus Latent TGF-Beta 1 (C33S), His Tag (Cat. No. TG1-C5243) at 2 μg/mL (100 μL/well) can bind Biotinylated Human ITGAV&ITGB8 Heterodimer Protein, His,Avitag&Tag Free (Cat. No. IT8-H82W5) with a linear range of 5-78 ng/mL (Routinely tested).
Protocol
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最新の進展
医薬品開発の進捗- 英語名：
Transforming growth factor beta 1
- カテゴリ：
- 市販の医薬品数：
1 詳細
- 治験医薬品数：
22 詳細
- 最新の開発ステージ：
Approved
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