Human MAG / Siglec-4a Protein, Fc Tag

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製品番号/仕様
価格
数量
MAG-H5254-100ug
$340.00
MAG-H5254-1mg
$2245.00
ETA of in-stock products:2 business days
総アイテム数 製品金額$ 0

製品詳細

  • Synonyms

    MAG, Siglec-4a, GMA, S-MAG

  • Source

    Human MAG, Fc Tag (MAG-H5254) is expressed from human 293 cells (HEK293). It contains AA Gly 20 - Pro 516 (Accession # P20916-1).

    Predicted N-terminus: Gly 20

    Request for sequence
  • Molecular Characterization

    MAG / Siglec-4a Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 81.1 kDa. The protein migrates as 100-115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Shipping and Storage

    This product is shipped at ambient temperature.

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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データ表示

  • SDS-PAGE

    MAG / Siglec-4a SDS-PAGE

    Human MAG, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

  • Bioactivity-SPR

     MAG / Siglec-4a SPR

    Human Nogo Receptor, His Tag (Cat. No. NOR-H52H3) capture on NTA-Series S sensor chip can bind Human MAG, Fc Tag (Cat. No. MAG-H5254) with an affinity constant of 2.21 μM as determined in a SPR assay (Biacore 8K) (Routinely tested).

    Protocol

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バックグランド

Myelin-associated glycoprotein (MAG), a nervous system cell adhesion molecule, is an I-type lectin that binds to sialylated glycoconjugates, including gangliosides bearing characteristic structural determinants. Preferentially binds to alpha-2,3-linked sialic acid. Binds ganglioside Gt1b. Adhesion molecule that mediates interactions between myelinating cells and neurons by binding to neuronal sialic acid-containing gangliosides and to the glycoproteins RTN4R and RTN4RL2. Protection against apoptosis is probably mediated via interaction with neuronal RTN4R and RTN4RL2. In dorsal root ganglion neurons the inhibition is mediated primarily via binding to neuronal RTN4R or RTN4RL2 and to a lesser degree via binding to neuronal gangliosides. In cerebellar granule cells the inhibition is mediated primarily via binding to neuronal gangliosides. In sensory neurons, inhibition of neurite extension depends only partially on RTN4R, RTN4RL2 and gangliosides.

最新の進展

 
医薬品開発の進捗
  • 英語名:

    Myelin-associated glycoprotein

  • カテゴリ:

  • 市販の医薬品数:

    0 詳細

  • 治験医薬品数:

    1 詳細

  • 最新の開発ステージ:

    Phase 2 Clinical

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