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製品詳細
Synonyms
CHDS6
Source
Human MMP-3, His Tag (MM3-H52H3) is expressed from human 293 cells (HEK293). It contains AA Tyr 18 - Thr 272 (Accession # P08254-1).
Predicted N-terminus: Tyr 18
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 30.7 kDa. The protein migrates as 32 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
Formulation
Supplied as 0.2 μm filtered solution in 25 mM MES, 150 mM NaCl, pH6.0 with glycerol as protectant.
Contact us for customized product form or formulation.
Shipping and Storage
This product is supplied and shipped on dry ice.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- The product MUST be stored at -20°C or lower upon receipt;
- -20°C for 3 months under sterile conditions.
ACRO Quality Management System
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データ表示
SDS-PAGE
Human MMP-3, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Human MMP-3, His Tag (Cat. No. MM3-H52H3) is more than 85% and the molecular weight of this protein is around 30-43 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human MMP-3, His Tag (Cat. No. MM3-H52H3) at 1 μg/mL (100 μL/well) can bind various dilution ratio of Anti-MMP3 Polyclonal Antibody (Routinely tested).
Protocol
Bioactivity
Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 . The specific activity is >422 pmol/min/µg (QC tested).
Protocol
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最新の進展
医薬品開発の進捗- 英語名：
Matrix metalloproteinase 3
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- 最新の開発ステージ：
Preclinical
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