SARS-CoV-2 (COVID-19) Papain-like Protease Protein, His Tag (active enzyme)

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製品番号/仕様
価格
数量
PAE-C5148-100ug
$465.00
PAE-C5148-1mg
$3400.00
ETA of in-stock products:2 business days
総アイテム数 製品金額$ 0

製品詳細

  • Synonyms

    Papain-like Protease, Replicase polyprotein 1a, ORF1a polyprotein, pp1a, 1a, PL-PRO, Plpro

  • Source

    SARS-CoV-2 Papain-like Protease, His Tag (PAE-C5148) is expressed from E. coli cells. It contains AA Glu 746 - Lys 1060 (Accession # YP_009725299.1).

    Predicted N-terminus: Met

    Request for sequence
  • Molecular Characterization

    Papain-like Protease Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the N-terminus.

    The protein has a calculated MW of 37.6 kDa. The protein migrates as 35-37 kDa under reducing (R) condition (SDS-PAGE).

  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Supplied as 0.2 μm filtered solution in 50 mM HEPES, 300 mM NaCl, pH7.5, Trehalose with glycerol as protectant.

    Contact us for customized product form or formulation.

  • Shipping and Storage

    This product is supplied and shipped on dry ice.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. The product MUST be stored at -70°C or lower upon receipt;
    2. -70°C for 3 months under sterile conditions.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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データ表示

  • SDS-PAGE

    Papain-like Protease SDS-PAGE

    SARS-CoV-2 Papain-like Protease, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

  • Bioactivity

    Measured by its ability to convert the substrate Dabcyl–FRLKGGAPIKGV–Edans.The specific activity is >2500 pmol/min/mg (QC tested).

    Protocol

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バックグランド

New coronavirus is a single-stranded RNA positive-strand envelope type B coronavirus. Similar to the other two coronaviruses that cause SARS (Severe Acute Respiratory Syndrome) and MERS (Middle East Respiratory Syndrome), its genome encodes non-structural, structural, and accessory proteins. Non-structural proteins include 3-chymotrypsin-like protease (3CLpro), papain-like protease, helicase, and RNA-dependent RNA polymerase (RNA -dependent RNA polymerase (RdRp). Structural proteins include spike glycoproteins. Papain in coronavirus is the same as 3CLpro. This enzyme operates on no less than 11 cleavage sites on the large polyprotein 1ab. Processing of polyproteins translated from viral RNA is essential. Therefore, press this The activity of this enzyme will prevent virus imitation.

最新の進展

 
医薬品開発の進捗
  • 英語名:

    SARS-CoV-2-3C-like-proteinase

  • カテゴリ:

  • 市販の医薬品数:

    6 詳細

  • 治験医薬品数:

    28 詳細

  • 最新の開発ステージ:

    Approved

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