Bat SARS-like coronavirus isolate Rs7327 Spike Trimer Protein (R668A, KV969-970PP), His Tag (MALS verified)

Synonyms

Spike, S protein, Spike glycoprotein, S glycoprotein

Source

Bat SARS-like coronavirus isolate Rs7327 Spike Trimer Protein, His Tag (SPN-S5227) is expressed from human 293 cells (HEK293). It contains AA Tyr 15 - Pro 1196 (Accession # ATO98218.1 (R668A, KV969-970PP)).

Predicted N-terminus: Tyr 15

Molecular Characterization

Other Tags and Version Biotin & Other Labeled Version

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 136.4 kDa. The protein migrates as 130-160 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Purity

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

Formulation

Lyophilized from 0.22 μm filtered solution in 0.1 M Sodium citrate, pH5.5 with trehalose as protectant.

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Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;
2. -70°C for 3 months under sterile conditions after reconstitution.

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