Mouse TNFR2 / CD120b / TNFRSF1B Protein, His Tag

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製品番号/仕様
価格
数量
TN2-M52H8-50ug
$270.00
TN2-M52H8-1mg
$2040.00
ETA of in-stock products:2 business days
総アイテム数 製品金額$ 0

製品詳細

  • Synonyms

    TNFRSF1B, CD120b, TBPII, TNF-R-II, TNF-R75, TNFBR, TNFR1B, TNFR2, TNFR80, p75, p75TNFR

  • Source

    Mouse TNFR2, His Tag (TN2-M52H8) is expressed from human 293 cells (HEK293). It contains AA Val 23 - Gly 258 (Accession # P25119-1).

    Predicted N-terminus: Val 23

    Request for sequence
  • Molecular Characterization

    TNFR2 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 27.2 kDa. The protein migrates as 45-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Shipping and Storage

    This product is shipped at ambient temperature.

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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データ表示

  • SDS-PAGE

    TNFR2 SDS-PAGE

    Mouse TNFR2, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

  • Bioactivity-ELISA

     TNFR2 ELISA

    Immobilized Mouse TNFR2, His Tag (Cat. No. TN2-M52H8) at 5 μg/mL (100 μL/well) can bind Biotinylated Human TNF-alpha, His,Avitag (active trimer) (MALS verified) (Cat. No. TNA-H82E3) with a linear range of 5-156 ng/mL (QC tested).

    Protocol
  •  TNFR2 ELISA

    Immobilized Mouse TNFR2, His Tag (Cat. No. TN2-M52H8) at 5 μg/mL (100 μL/well) can bind Biotinylated Human TNF-alpha, epitope tag free, primary amine labeling (Cat. No. TNA-H8211) with a linear range of 2-39 ng/mL (Routinely tested).

    Protocol

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バックグランド

TNF RI is also known as the p60 or p55 TNFR) and TNF RII (the p75 or p80 TNFR) are two distinct type I transmembrane glycoproteins that bind TNF with highaffinity.Both RI and RII are prototypic members of the TNF receptor superfamily and have been designated TNFRSF1A and TNFRSF1B, respectively. Human TNF RII cD encodes a 461 amino acid (aa) residue precursor protein with a 22 aa putative signal peptide, a 235 aa extracellular domain, a 20 aa transmembrane domain and a 174 aa cytoplasmic domain. TNFRII is expressed in fetal brain. The protein is produced naturally as a soluble form (sTNFRII). The soluble receptor inhibits TNFα action by competing with cell surface receptors in binding TNFα, thereby blocking its biologic effects. TNFRII is strongly expressed at the cartilage–pannus junction, and plays a major role in a subset of families with multiple cases of rheumatoid arthritis (RA). Further, high plasma levels of sTNFRII were significantly associated with increased incidence of coronary heart disease, independent of established cardiovascular risk factors, and seems to be useful for monitoring the inflammatory activity of sarcoidosis.

最新の進展

 
医薬品開発の進捗
  • 英語名:

    Tumor necrosis factor receptor superfamily member 1B

  • カテゴリ:

  • 市販の医薬品数:

    1 詳細

  • 治験医薬品数:

    8 詳細

  • 最新の開発ステージ:

    Approved

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