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Your Position: ホーム > Protein > E8L > E8L-M52H3

Monkeypox virus (strain Zaire-96-I-16) E8L Protein, His Tag

  • Synonym
    E8L
  • Source
    Monkeypox virus (strain Zaire-96-I-16) E8L, His Tag(E8L-M52H3) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Thr 275 (Accession # Q8V4Y0-1 ).
    Predicted N-terminus: Met 1
  • Molecular Characterization
    E8L Structure

    This protein carries a polyhistidine tag at the C-terminus

    The protein has a calculated MW of 33.7 kDa. The protein migrates as 40-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
E8L SDS-PAGE

Monkeypox virus (strain Zaire-96-I-16) E8L, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA
 E8L ELISA

Immobilized Monkeypox virus (strain Zaire-96-I-16) E8L, His Tag (Cat. No. E8L-M52H3) at 5 μg/mL (100 μL/well) can bind Anti-Monkeypox-E8L-Antibody with a linear range of 0.1-3 ng/mL (QC tested).

  • Background
    Monkeypox is a rare zoonosis caused by monkeypox virus, which has become the most serious orthpoxvirus and consists of complex double stranded DNA. The cases are mostly in central and western Africa. The pathogenesis of monkeypox is that the virus invades the body from respiratory mucosa , multiplies in lymphocytes, and incurs into blood producing transient venereal toxemia. after the virus multiplies in cells, the cells can invade the blood and propagate to the skin of the whole body, causing lesions. E8L can Binds to chondroitin sulfate on the cell surface to provide virion attachment to target cell.
  • Clinical and Translational Updates

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