Monkeypox virus (strain Zaire-96-I-16) B21R Protein, His Tag

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  • Product Details
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分子别名(Synonym)

B21R

表达区间及表达系统(Source)

Monkeypox virus (strain Zaire-96-I-16) B21R , His Tag (B2R-M52H3) is expressed from human 293 cells (HEK293). It contains AA Met 1 - His 1816 (Accession # Q8V4Q7).

Predicted N-terminus: Met 1

蛋白结构(Molecular Characterization)

B21R Structure

This protein carries a polyhistidine tag at the C-terminus

The protein has a calculated MW of 206.5kDa.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.

Background

Monkeypox is a rare zoonosis caused by monkeypox virus, which has become the most serious orthpoxvirus and consists of complex double stranded DNA. The cases are mostly in central and western Africa. The pathogenesis of monkeypox is that the virus invades the body from respiratory mucosa, multiplies in lymphocytes, and incurs into blood producing transient venereal toxemia. after the virus multiplies in cells, the cells can invade the blood and propagate to the skin of the whole body, causing lesions. The important target protein B21R with key immune dominant epitopes is considered to be the focus of monkeypox virus research.