Recombinant Monoclonal Anti-B7-H3 Antibody, Rabbit (7A1)

製品詳細

Background
Recombinant Monoclonal Anti-B7-H3 Antibody, Rabbit (7A1) is a B7-H3 (also called CD276) primary antibody for IHC application. B7-H3 is over-expressed in a variety of tumors in the lung, pancreas, breast, colon and prostate.

Host Species	Rabbit
Isotype	Rabbit IgG, Rabbit Kappa
Clone	7A1
Application	IHC-P
Dilution Ratio	1: 3000 - 1: 7000
Form	Liquid
Clonality	Monoclonal
Reactivity	Human
Synonyms	CD276
Concentration	0.9~1.1 mg/ml
Specificity	React with B7-H3 protein on the membrane of target cell/tissue
Purification Tech	Protein A
Preservation	PBS, BSA, Glycerol

Experiment Protocol
LEICA Experiment Protocol
Storage
Shipped at 4°C, Blue Ice. Store at +4°C for short term (1-2 weeks). Store at -20°C for long term.
Avoid freeze / thaw cycle. Upon delivery aliquot.
ACRO Quality Management System

Sample Staining Data
Immunohistochemical analysis of paraffin embedded human prostate cancer tissue labelled with HGS-S609 at 1:5000 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with LEICA BOND IHC staining protocol.
Immunohistochemical analysis of paraffin embedded human ovarian cancer tissue labelled with HGS-S609 at 1:5000 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with LEICA BOND IHC staining protocol.
Immunohistochemical analysis of paraffin embedded human colorectal cancer tissue labelled with HGS-S609 at 1:5000 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with LEICA BOND IHC staining protocol.