Biotinylated FMC63 scFv, Fc,Avitag™ (MALS verified)

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Cat. No. / Size
Price
Qty
CD9-BV4324b-25ug
$280.00
CD9-BV4324b-200ug
$1420.00
ETA of in-stock products:2 business days

Product Details

  • Source

    Biotinylated FMC63 scFv, Fc, Avitag™ is a recombinant single-chain variable fragment derived from the mouse monoclonal antibody clone FMC63 and expressed in HEK293 cells.

  • Antibody Type

    Recombinant Monoclonal

  • Host Species

    Mouse

  • Reactivity

    Human

  • Immunogen

    CD19

  • Conjugate

    Biotin

  • Specificity

    Specifically recognizes the CD19.

  • Application

    Application
    Recommended Usage
    ELISA
    1:5-1:50
  • Purification

    Affinity purification.

  • Aggregation

    Less than 5%, as determined by SEC-MALS.

  • Concentration

    Please refer to the Certificate of Analysis (CoA).

  • Form

    Lyophilized

  • Formulation

    Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4), with trehalose as protectant.

    Please contact us for customized product forms or formulations.

  • Reconstitution

    Please refer to the Certificate of Analysis (CoA) for specific instructions.

  • Shipping

    Lyophilized product is shipped at ambient temperature.

  • Storage

    For long term storage, the product should be stored in a lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • Notices

    Product Specific Notices: For research use only.

  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • Bioactivity-ELISA

     CD19 ELISA

    A microplate was coated with FMC63 monoclonal antibody, Mouse IgG2a, at 1 μg/mL. Serially diluted Rabbit Anti-Mouse FMC63 Polyclonal Antibody was added, followed by Biotinylated FMC63 scFv-Fc-Avitag at 2 μg/mL. Binding was detected using HRP-conjugated streptavidin. The QC-tested sensitivity was 78 ng/mL (QC tested).

    Protocol

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Background

FMC63 is an IgG2a mouse monoclonal antibody specific for CD19, which is a target for the immunotherapy of B lineage leukaemias and lymphomas. FMC63 scFv is the most commonly used ectodomain component of CD19-specific CARs. So far, most of reported CART19 trials contain the anti-CD19 scFv derived from FMC63, including the two FDA-approved CARs Kymriah and Yescarta.

Recent Advances

 
Drug Development Progress
  • English Name:

    B-lymphocyte antigen CD19

  • Category:

  • Approved Drugs:

    14 Details

  • Drugs in Clinical Trials:

    435 Details

  • Highest Development Stage:

    Approved

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