PE-Labeled Monoclonal Anti-Human CD223 / LAG-3 Antibody, Mouse IgG1 (AS659) (0.03% Proclin)

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FABm061-01-50tests
$240.00
FABm061-01
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Source

Monoclonal Anti-Human CD223 / LAG-3 Antibody, Mouse IgG1 (AS659) is a mouse monoclonal antibody, which provides higher batch consistency and long term security of supply.

Clone

AS659

Isotype

Mouse IgG1, Kappa

Host Species

Mouse

Reactivity

Human

Immunogen

Purified Human CD223 / LAG-3 epsilon Protein

Conjugate

PE

Excitation Wavelength: 488 nm / 561 nm

Emission Wavelength: 575 nm

Specificity

This product is a specific antibody specifically reacts with CD223 / LAG-3 epsilon protein.

Application
Application
Recommended Use
Flow Cytometry
1:20

* Recommended dilutions are for reference only. Optimal working concentrations may vary depending on your consumables, reagents, and assay conditions. We recommend optimizing for your specific system. No warranty is made regarding performance at any specific dilution.

Isotype Control

The isotype control is sold separately; please refer to Cat. No. DNP-PM1 for product information.

Concentration

Please refer to the Certificate of Analysis (CoA).

Form

Liquid

Formulation

Supplied a 0.22 μm-filtered solution in PBS (pH 7.4) containing 0.03% ProClin 300 and 0.2% BSA, with trehalose as protectant.

Please contact us for customized product forms or formulations.

Shipping

Liquid product is shipped at blue ice.

Storage

Please protect from light and avoid repeated freeze-thaw cycles.

This product is stable after storage at:
  • Store at 2-8 °C for 12 months.
Notices

Product Specific Notices: For research use only.

バックグランド

This PE-conjugated anti-human CD223 (LAG-3) antibody specifically recognizes CD223 (Lymphocyte Activation Gene 3/Protein FDC), a high-performance tool for immunoregulatory research. CD223 (LAG-3) is a ~70 kDa Ig superfamily transmembrane glycoprotein, homologous to CD4, critical for T-cell signaling. Expressed on activated T cells, NK cells and Tregs (higher on CD8⁺ than CD4⁺ T cells), transient in acute activation but sustained under tolerance. As an activation-induced molecule, CD223 binds MHC class II with higher affinity than CD4 (competitive inhibition), associates with TCR-CD3 to downregulate signaling and T-cell expansion (negative T-cell regulation), and promotes dendritic cell activation. CD223 deficiency inhibits tumor growth; synergizes with PD-1 to reverse T-cell exhaustion, enhance tumor rejection and control viral loads. This antibody facilitates CD223 detection, immune mechanism study and tumor immunotherapy research.

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