Recombinant Monoclonal Anti-CD45RO Antibody, Mouse (4C8)

製品詳細

Background
Recombinant Monoclonal Anti-CD45RO Antibody, Mouse (4C8) is a CD45RO primary antibody for IHC application. CD45RO (UCHL1, ubiquitin carboxyl terminal esterase L1) is the shortest isoform of a receptor-type protein tyrosine phosphatase, CD45 glycoprotein. CD45 is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases, promotes cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis.

Host Species	Mouse
Isotype	Mouse IgG
Clone	4C8
Application	IHC-P
Dilution Ratio	1: 200 - 1: 1000
Form	Liquid
Clonality	Monoclonal
Reactivity	Human
Synonyms	CD45RO
Concentration	0.9~1.1 mg/ml
Specificity	React with CD45RO protein on the membrane of target cell/tissue
Purification Tech	Protein A
Preservation	Tris buffer, 1% BSA

Experiment Protocol
LEICA Experiment Protocol
Storage
Shipped at 4°C, Blue Ice. Store at +4°C for short term (1-2 weeks). Store at -20°C for long term.
Avoid freeze / thaw cycle. Upon delivery aliquot.
ACRO Quality Management System

Sample Staining Data
Immunohistochemical analysis of paraffin embedded human cancer tissue labelled with HGS-S249 at 1:500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.