Recombinant Monoclonal Anti-CD5 Antibody, Rabbit (4G5)

製品詳細

Background
Recombinant Monoclonal Anti-CD5 Antibody, Rabbit (4G5) is a CD5 primary antibody for IHC application. CD5 is present on all mature T-lymphocytes, on most of thymocytes and on many T-cell leukemias and lymphomas. CD5 also reacts with a subpopulation of activated B-cells and may act as a receptor in regulating T-cell proliferation. CD5 is found on 95% of thymocytes and 72% of peripheral blood lymphocytes. In lymph nodes, the main reactivity is observed in T cell areas. CD5 is expressed by many T cell leukemia, lymphomas, and activated T cells. Diseases associated with CD5 dysfunction include thymus cancer and Richter's Syndrome.

Host Species	Rabbit
Isotype	Rabbit IgG, Rabbit Kappa
Clone	4G5
Application	IHC-P
Dilution Ratio	1: 500 - 1: 2000
Form	Liquid
Clonality	Monoclonal
Reactivity	Human
Synonyms	Leu-1
Concentration	0.9~1.1 mg/ml
Specificity	React with CD5 protein on the membrane of target cell/tissue
Purification Tech	Protein A
Preservation	Tris buffer, 1% BSA

Experiment Protocol
LEICA Experiment Protocol
Storage
Shipped at 4°C, Blue Ice. Store at +4°C for short term (1-2 weeks). Store at -20°C for long term.
Avoid freeze / thaw cycle. Upon delivery aliquot.
ACRO Quality Management System

Sample Staining Data
Immunohistochemical analysis of paraffin embedded human cancer tissue labelled with HGS-S250 at 1:1000 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.