PE-Labeled Monoclonal Anti-Human CD69 Antibody, Mouse IgG1 (3C5B7) (0.03% Proclin)

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FABm022-01-50tests
$100.00
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$200.00
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Source

PE-Labeled Monoclonal Anti-Human CD69 Antibody, Mouse IgG1 (3C5B7) is a mouse monoclonal antibody recombinantly expressed from Hybridoma.

Antibody Type

Hybridoma Monoclonal

Clone

3C5B7

Isotype

Mouse IgG1, Kappa

Host Species

Mouse

Reactivity

Human

Immunogen

Purified Human CD69 Protein

Conjugate

PE

Excitation Wavelength: 488 nm / 561 nm

Emission Wavelength: 575 nm

Specificity

This product is a specific antibody specifically reacts with CD69 protein.

Application

Flow Cytometry (Evaluation of the expression of CD69 on Human cells).

Recommended Dilution

1:20

Isotype Control

The isotype control is sold separately; please refer to Cat. No. DNP-PM1 for product information.

Form

Liquid

Formulation

Supplied as 0.2 μm-filtered solution in PBS (pH 7.4) containing 0.03% ProClin 300 and 0.2% BSA, with trehalose as protectant.

Please contact us for customized product forms or formulations.

Shipping

Liquid product is shipped at blue ice.

Storage

Please protect from light and avoid repeated freeze-thaw cycles.

This product is stable after storage at:
  • Store at 2-8 °C for 12 months.
Notices

Product Specific Notices: For research use only.

Background

The activation of T lymphocytes involves a wide variety of cellular changes. Some of these changes are biochemical in nature, such as phosphatidylinositol catabolism and the elevationof cellular calcium. Others are genetic, involving the induction of specific gene expression. Among the constellation of genes induced following T cell activation are several cell surface molecules. One of the earliest of these to be expressed is CD69 (previously known as activation inducer molecule or AIM, EA-1, Leu-23,and MLR3). Within one to two h of activation, CD69 can be found on the cell surface, with expression persisting at least three days. Studies with monoclonal antibodies (MAb) to CD69 have shown that it is a costimulatory molecule for T cell proliferation. In addition, CD69 expression can be induced on most, if not all, cells of hematopoietic origin.