Biotinylated Anti-Cetuximab Antibody (AY31) (recommended for PK/PD)

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Cat. No. / Size
Price
Qty
CEB-BY31-25ug
$435.00
CEB-BY31-200ug
$1975.00
ETA of in-stock products:2 business days

Product Details

  • Source

    Biotinylated Anti-Cetuximab Antibody (AY31) is a mouse monoclonal antibody derived from hybridoma cells fused with SP2/0 myeloma cells and murine B lymphocytes.

  • Antibody Type

    Hybridoma Monoclonal

  • Clone

    AY31

  • Isotype

    Mouse IgG1, Kappa

  • Host Species

    Mouse

  • Immunogen

    Cetuximab

  • Conjugate

    Biotin

  • Specificity

    Recognizes Cetuximab specifically.

  • Application

    Application
    Recommended Usage
    ELISA
    1:300-1:3000
  • Purification

    Protein A purified / Protein G purified.

  • Concentration

    Please refer to the Certificate of Analysis (CoA).

  • Form

    Lyophilized

  • Formulation

    Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4) containing 0.03% PF68, with trehalose as protectant.

    Please contact us for customized product forms or formulations.

  • Reconstitution

    Please refer to the Certificate of Analysis (CoA) for specific instructions.

  • Shipping

    Lyophilized product is shipped at ambient temperature.

  • Storage

    For long term storage, the product should be stored in a lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • Notices

    Product Specific Notices: For research use only.

  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • Bioactivity-ELISA

     Cetuximab ELISA

    Immobilized Anti-Cetuximab Antibody (12B1E9) (Cat. No.CEB-Y29c) at 2 μg/mL, add increasing concentrations of Cetuximab and then add Biotinylated Anti-Cetuximab Antibody (AY31) (Cat. No.CEB-BY31) at 1 μg/mL. Detection was performed using HRP-conjugated streptavidin with sensitivity of 4.84 ng/mL (QC tested).

    Protocol
  •  Cetuximab ELISA

    Immobilized cetuximab at 0.1 μg/mL (100 μL/well) can bind Biotinylated Anti-Cetuximab Antibody (AY31) (Cat. No. CEB-BY31) with a linear range of 0.39-25 ng/mL.

    Protocol
  •  Cetuximab ELISA

    ELISA analysis shows that the binding of cetuximab to Human EGF R, His Tag (Cat. No. EGR-H5222) was inhibited by increasing concentration of Biotinylated Anti-Cetuximab Antibody (AY31) (Cat. No. CEB-BY31). The concentration of cetuximab used is 3 ng/mL.

    Protocol

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Background

Cetuximab is an epidermal growth factor receptor binding FAB. Cetuximab is composed of the Fv (variable; antigen-binding) regions of the 225 murine EGFr monoclonal antibody specific for the N-terminal portion of human EGFr with human IgG1 heavy and kappa light chain constant (framework) regions.

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