Product Details
Source
Anti-Cetuximab Antibody (AY31) is a mouse monoclonal antibody derived from hybridoma cells fused with SP2/0 myeloma cells and murine B lymphocytes.
Antibody Type
Hybridoma Monoclonal
Clone
AY31
Isotype
Mouse IgG1, Kappa
Host Species
Mouse
Immunogen
Cetuximab
Specificity
Recognizes Cetuximab specifically.
Purification
Protein A purified / Protein G purified.
Concentration
Please refer to the Certificate of Analysis (CoA).
Form
Lyophilized
Formulation
Lyophilized from a 0.22 μm-filtered solution in Tris (pH 7.5) containing 100 mM Glycine, 25 mM Arginine and 150 mM NaCl, with trehalose as protectant.
Please contact us for customized product forms or formulations.
Reconstitution
Please refer to the Certificate of Analysis (CoA) for specific instructions.
Shipping
Lyophilized product is shipped at ambient temperature.
Storage
For long term storage, the product should be stored in a lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Notices
Product Specific Notices: For research use only.
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Performance Data
Bioactivity-ELISA

Anti-Cetuximab Antibodies bridging ELISA for Anti-Drug Antibody (ADA) assay development.Immobilized cetuximab at 1 µg/mL, add increasing concentrations of Anti-Cetuximab Antibody (AY31) (Cat. No. CEB-Y31, 10% human serum) and then add biotinylated cetuximab at 2 µg/mL. Detection was performed using HRP-conjugated streptavidin with a sensitivity of 39 ng/mL (QC tested).
Protocol
Demonstration of the specificity of Anti-Cetuximab Antibody (AY31) (Cat. No. CEB-Y31) to the cetuximab.
Protocol
Immobilized cetuximab at 2 μg/mL (100 μL/well) can bind pre-mixed Anti-Cetuximab Antibody (AY31) (Cat. No. CEB-Y31) and Biotinylated Human EGFRvIII, His,Avitag (Cat. No. EGR-H82E0) with a inhibition rate of 0%.
Protocol
Bioactivity-SPR

Anti-Cetuximab Antibody (AY31) (mouse IgG1, Cat. No. CEB-Y31) captured on CM5 chip via anti-mouse antibodies surface, can bind human cetuximab with an affinity constant of 0.421 nM.
Protocol
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