Recombinant Monoclonal Anti-E-Cadherin Antibody, Mouse (3F7)

製品詳細

Background
Recombinant Monoclonal Anti-E-Cadherin Antibody, Mouse (3F7) is a E-Cadherin primary antibody for IHC application. E-Cadherin plays a critical role in cell-cell juncations. Due to these vital functions, it serves important roles in both physiological and pathological contexts, including the tumor metastasis and invasion. E-Cadherin is over-expressed in a variety of tumors so that it is widely employed in cell biology and oncology research.

Host Species	Mouse
Isotype	Mouse IgG
Clone	3F7
Application	IHC-P
Dilution Ratio	1: 100 - 1: 500
Form	Liquid
Clonality	Monoclonal
Reactivity	Human
Synonyms	Cadherin-1
Concentration	0.9~1.1 mg/ml
Specificity	React with E-Cadherin protein on the membrane of target cell/tissue
Purification Tech	Protein A
Preservation	Tris buffer, 1% BSA

Experiment Protocol
LEICA Experiment Protocol
Storage
Shipped at 4°C, Blue Ice. Store at +4°C for short term (1-2 weeks). Store at -20°C for long term.
Avoid freeze / thaw cycle. Upon delivery aliquot.
ACRO Quality Management System

Sample Staining Data
Immunohistochemical analysis of paraffin embedded human breast cancer tissue labelled with HGS-S590 at 1:100 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with LEICA BOND IHC staining protocol.
Immunohistochemical analysis of paraffin embedded human ovarian cancer tissue labelled with HGS-S590 at 1:100 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with LEICA BOND IHC staining protocol.