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製品詳細
Source
Monoclonal Anti-G4S linker Antibody, Rabbit IgG (016) is a rabbit monoclonal antibody recombinantly expressed from human 293 cells (HEK293), which provides higher batch consistency and long term security of supply. It shows superior performance in flow cytometry assays with higher affinity and better specificity.
Antibody Type
Recombinant Monoclonal
Clone
016
Isotype
Rabbit IgG, Kappa
Host Species
Rabbit
Conjugate
AF488
Excitation Wavelength: 488 nm
Emission Wavelength: 517 nm
Specificity
It can specifically recognize proteins or ScFv containing (G3S)n or (G4S)n linkers, where n = 2, 3, 4... That is, it can recognize linkers with a minimum length of 2 repeats for both (G3S)n and (G4S)n.
Application
Nanoflow cytometry (tLNP conjugation ratio detection).
Flow Cytometry (Evaluation of cell surface expressed CARs of varying specificity containing a G4S linker within the scFv of the extracellular domain).
Recommended Dilution
1:50
Form
Lyophilized
Formulation
Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4) containing 0.03% ProClin 300, with trehalose as protectant.
Please contact us for customized product forms or formulations.
Reconstitution
Please refer to the Certificate of Analysis (CoA) for specific instructions.
Shipping
Lyophilized product is shipped at ambient temperature.
Storage
For long term storage, the product should be stored in a lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 24 months in lyophilized state;
- -70°C for 12 months after reconstitution.
- 2-8 °C for 12 months after reconstitution.
Notices
Product Specific Notices: For research use only.
ACRO Quality Management System
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Bioactivity-FACS
Flow cytometric analysis of Anti-MSLN CAR-293 cells staining with Alexa Fluor 488-Labeled Monoclonal Anti-G4S linker Antibody, Rabbit IgG (016) (Cat. No. G4S-AFYP1) at 1:50 dilution (2 μL of the antibody stock solution corresponds to labeling of 1e6 cells in a final volume of 100 µL), compared with Isotype control antibody. Alexa Fluor 488 signal was used to evaluate the binding activity (QC tested).
Protocol
Non-specificity of Alexa Fluor 488-Labeled Monoclonal Anti-G4S linker Antibody, Rabbit IgG (016) (Cat. No. G4S-AFYP1) binding to CD3+ cells present in human PBMC. 5e5 of human PBMCs were simultaneously stained with PE anti-CD3 antibody and Alexa Fluor 488-Labeled Monoclonal Anti-G4S linker Antibody, Rabbit IgG (016) (2 μL of the antibody stock solution corresponds to labeling of 5e5 cells in a final volume of 100 µL) and washed and then analyzed with FACS. Both PE and Alexa Fluor 488 positive signals was used to evaluate the non-specific binding activity to human CD3+ cells (QC tested).
Protocol
Nanoflow Cytometry (tLNP)
2-3*10¹⁰ particles/mL of tLNP (Coupled with scFv containing G4S linker) were stained with 3 μg/mL of Alexa Fluor™ 488-Labeled Monoclonal Anti-G4S linker Antibody, Rabbit IgG (016) (Cat. No. G4S-AFYP1), AF488 signal was exploited to quantify tLNP conjugation ratio and surface antibody density (Routinely tested).
Protocol
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