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Product Details
Source
Monoclonal Anti-Influenza A (A/Hong Kong/483/1997) HA (H5N1) Antibody, Human IgG1 (1A4) is a recombinant chimeric monoclonal antibody expressed in HEK293, which combines the variable region of a mouse monoclonal antibody with human constant domain.
Antibody Type
Recombinant Monoclonal
Clone
1A4
Isotype
Human IgG1, Kappa
Host Species
Mouse
Reactivity
Influenza A
Immunogen
Recombinant Influenza A [A/Hong Kong/483/97 (H5N1)] HA is expressed from human 293 cells
Specificity
Specifically recognizes Influenza A [A/Hong Kong/483/97 (H5N1)] Hemagglutinin (HA) Protein.
Application
ApplicationRecommended UsageWestern Blot2-0.1 ug/mLELISA0.2-2 μg/mLCross Verification
This product No cross-reactivity in ELISA with
Influenza A [A/Darwin/6/2021 (H3N2)] HA Protein, His Tag (Cat. No. HA2-V52H5).
Influenza A [A/Darwin/9/2021 (H3N2)] HA Protein, His Tag (Cat. No. HA2-V52H6).
Influenza A (A/Shanghai/02/2013(H7N9)) Hemagglutinin (HA) Protein, His Tag (Cat. No. HA9-V52H3).
Influenza B [Austria/1359417/2021 (B/Victoria lineage)] Hemagglutinin (HA) Protein, His Tag (Cat. No. HAE-V52H3).
Influenza B [Phuket/3073/2013 (B/Yamagata lineage)] HA Protein, His Tag (Cat. No. HAE-V52H4).
Influenza A [A/Bangkok/1/1979 (H3N2)] HA, His Tag (Cat. No. HA2-V52H3).
Influenza A Virus HA (H3N2) Protein, His Tag (Cat. No. H32-V52H3).Purification
Protein A purified / Protein G purified.
Aggregation
Less than 10%, as determined by SEC-MALS.
Concentration
Please refer to the Certificate of Analysis (CoA).
Form
Lyophilized
Formulation
Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4), with trehalose as protectant.
Please contact us for customized product forms or formulations.
Reconstitution
Please refer to the Certificate of Analysis (CoA) for specific instructions.
Shipping
Lyophilized product is shipped at ambient temperature.
Storage
For long term storage, the product should be stored in a lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Notices
Product Specific Notices: For research use only.
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Performance Data
Bioactivity-ELISA
Immobilized Influenza A [A/Hong Kong/483/97 (H5N1)] HA, His Tag (Cat. No. HA1-V5229) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Influenza A (A/Hong Kong/483/1997) HA (H5N1) Antibody, Human IgG1 (1A4) (Cat. No. HA1-MY2026) with a linear range of 0.1-4 ng/mL (QC tested).
Protocol
Immobilized Influenza A [Victoria/4897/2022] Hemagglutinin (HA) Protein, His Tag (Cat. No. HA1-V52H8) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Influenza A (A/Hong Kong/483/1997) HA (H5N1) Antibody, Human IgG1 (1A4) (Cat. No. HA1-MY2026) with a linear range of 0.2-3 ng/mL (Routinely tested).
Protocol
Immobilized Influenza A [Wisconsin/67/2022] Hemagglutinin (HA) Protein, His Tag (Cat. No. HA1-V52H7) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Influenza A (A/Hong Kong/483/1997) HA (H5N1) Antibody, Human IgG1 (1A4) (Cat. No. HA1-MY2026) with a linear range of 0.2-3 ng/mL (Routinely tested).
Protocol
Immobilized Influenza A [A/Victoria/2570/2019] Hemagglutinin (HA) Protein, His Tag (Cat. No. HA1-V52H6) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Influenza A (A/Hong Kong/483/1997) HA (H5N1) Antibody, Human IgG1 (1A4) (Cat. No. HA1-MY2026) with a linear range of 0.4-13 ng/mL (Routinely tested).
Protocol
Immobilized Influenza A [Sydney/5/2021 (H1N1)] HA Protein, His Tag (Cat. No. HA1-V52H4) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Influenza A (A/Hong Kong/483/1997) HA (H5N1) Antibody, Human IgG1 (1A4) (Cat. No. HA1-MY2026) with a linear range of 0.1-3 ng/mL (Routinely tested).
Protocol
Immobilized Influenza A (Vietnam/1194/2004(H5N1)) Hemagglutinin (HA) Protein, His Tag (Cat. No. HA1-V52H9) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Influenza A (A/Hong Kong/483/1997) HA (H5N1) Antibody, Human IgG1 (1A4) (Cat. No. HA1-MY2026) with a linear range of 0.1-3 ng/mL (Routinely tested).
Protocol
Immobilized Influenza A (Guangdong/18SF020(H5N6)) Hemagglutinin (HA) Protein, His Tag (Cat. No. HA6-V52H3) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Influenza A (A/Hong Kong/483/1997) HA (H5N1) Antibody, Human IgG1 (1A4) (Cat. No. HA1-MY2026) with a linear range of 0.2-3 ng/mL (Routinely tested).
Protocol
Immobilized Influenza A (turkey/Germany-MV/R2472/2014(H5N8)) HA Protein, His Tag (Cat. No. HA8-V52H3) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Influenza A (A/Hong Kong/483/1997) HA (H5N1) Antibody, Human IgG1 (1A4) (Cat. No. HA1-MY2026) with a linear range of 0.2-3 ng/mL (Routinely tested).
Protocol
Immobilized Influenza A [A/guinea fowl/Hong Kong/WF10/99(H9N2)] Hemagglutinin (HA) Protein, His Tag (Cat. No. HA2-V52H7) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Influenza A (A/Hong Kong/483/1997) HA (H5N1) Antibody, Human IgG1 (1A4) (Cat. No. HA1-MY2026) with a linear range of 0.2-3 ng/mL (Routinely tested).
Protocol
Immobilized Influenza A [A/Wisconsin/588/2019 (H1N1)] HA, His Tag (Cat. No. HA1-V52H3) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Influenza A (A/Hong Kong/483/1997) HA (H5N1) Antibody, Human IgG1 (1A4) (Cat. No. HA1-MY2026) with a linear range of 0.2-3 ng/mL (Routinely tested).
Protocol
Western Blot
Western blot analysis of Influenza A (turkey/Germany-MV/R2472/2014(H5N8)) HA Protein, His Tag (Cat. No. HA8-V52H3). The protein was loaded at 400 ng per lane and detected using Monoclonal Anti-Hemagglutinin/HA (Influenza Virus) (A/Hong Kong/483/1997 (H5N1)) Antibody, Human IgG1 (1A4) at 0.1 μg/mL, followed by HRP-conjugated Goat Anti-Human IgG, Fcγ fragment specific secondary antibody at a 1:2000 dilution. A specific band was detected at approximately 80 kDa.
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