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Product Specifications
SourceThis Monoclonal Anti-His Tag Antibody, Rabbit IgG is a chimeric monoclonal antibody recombinantly expressed from HEK293, which combines the variable region of a mouse monoclonal antibody with Rabbit constant domain.SpecificityThis antibody specifically reacts with His tag proteins, and can recognizes C-terminal, N-terminal, and internal His tagged fusion proteins.FormulationLyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.Physical AppearancePowderStorage (unopened)-20°C to -70°CStorage (opened)-70°CUnit Size1000 tests & 5000 tests & 20000 testsTechnologyTR-FRETIntended UseFor binding the his-tagged protein as Donor in TR-FRET AssayReconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
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Performance Data
Bioactivity-TR-FRET
Please refer to DS document for the assay protocol.
In this TR-FRET assay, Monoclonal Anti-His Tag Antibody Europium chelate (Cat. No. FRT-TD05) is used as the Donor and Streptavidin Protein FA-Labeled (Cat. No. FRT-TA01) is used as the Acceptor. Biotinylated Human PD-1, Fc,Avitag (Cat. No. PD1-H82F1), at 62.5 ng/well, can bind to the Human PD-L1, His Tag (Cat. No.PD1-H5229) with a linear detection range of 10-0.01 μg/mL (QC tested).
In this TR-FRET assay, Monoclonal Anti-His Tag Antibody Europium chelate (for TR-FRET) (Cat. No. FRT-TD05) is used as the Donor and Monoclonal Anti-Human IgG Antibody FA-Labeled (for TR-FRET) (Cat. No. FRT-TA02) is used as the Acceptor. Human TROP-2, His Tag (Cat. No. TR2-H5223), at 1.25 ng/well, can bind to the Datopotamab with a linear detection range of 8-0.001 μg/mL (Routinely tested).
In this TR-FRET assay, Monoclonal Anti-His Tag Antibody Europium chelate (for TR-FRET) (Cat. No. FRT-TD05) is used as the Donor and Monoclonal Anti-Mouse IgG Antibody FA-Labeled (for TR-FRET) (Cat. No. FRT-TA03) is used as the Acceptor. Human CD20 Full Length, His Tag (Cat. No. CD0-H52H1), at 5 ng/well and Human CD3 epsilon, Mouse IgG2a Fc Tag (Cat. No. CDN-H5253), at 2.5 ng/well, can bind to the Glofitamab with a linear detection range of 16-0.008 μg/mL (Routinely tested).
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