Alexa Fluor™ 488-Labeled Monoclonal Anti-His Antibody, Human IgG1 (55F8)

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HIS-AFM549-25tests
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Source

Monoclonal Anti-His Antibody, Human IgG1 (55F8) is a chimeric monoclonal antibody recombinantly expressed from HEK293, which combines the variable region of a mouse monoclonal antibody with Human constant domain, which provides higher batch consistency and long term security of supply.

Antibody Type

Recombinant Monoclonal

Clone

55F8

Isotype

Human IgG1, Kappa

Host Species

Mouse

Reactivity

His tag (N‑terminal, C‑terminal, internal His‑tagged fusion proteins)

Immunogen

A protein peptide with his tag

Conjugate

AF488

Excitation Wavelength: 488 nm

Emission Wavelength: 517 nm

Specificity

This product is a specific antibody specifically reacts with His tag protein, and can recognizes C-terminal, N-terminal, and internal His tagged fusion proteins.

Application

Flow Cytometry (Detection the His-tagged protein).

Recommended Dilution

1:50

Form

Lyophilized

Formulation

Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4), with trehalose as protectant.

Please contact us for customized product forms or formulations.

Reconstitution

Please refer to the Certificate of Analysis (CoA) for specific instructions.

Shipping

Lyophilized product is shipped at ambient temperature.

Storage

For long term storage, the product should be stored in a lyophilized state at -20°C or lower.

Please protect from light and avoid repeated freeze-thaw cycles.

This product is stable after storage at:
  • -20°C to -70°C for 24 months in lyophilized state;
  • -70°C for 12 months under sterile conditions after reconstitution.
Notices

Product Specific Notices: For research use only.

Background

His tag is a very small molecular weight tag, usually composed of 6-10 histidine (His). It is one of the commonly used tags for protein purification and detection. Due to its small molecular weight, fusion into the target protein has almost no effect on the structure and characteristics of the protein. Anti-his tag antibody can accurately detect, locate and purify His tag fusion protein, so as to provide convenience for the vast number of researchers.