PE-Labeled Monoclonal Anti-His Antibody, Mouse IgG1 (55F8)

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HIS-PFY63-25tests
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$370.00
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Source

PE-Labeled Monoclonal Anti-His Antibody, Mouse IgG1 (55F8) is a mouse monoclonal antibody recombinantly expressed from Hybridoma.

Antibody Type

Hybridoma Monoclonal

Clone

55F8

Isotype

Mouse IgG1, Kappa

Host Species

Mouse

Reactivity

His tag (N-terminal, C-terminal and internal His-tagged fusion proteins)

Immunogen

Purified his-tagged Protein

Conjugate

PE

Excitation Wavelength: 488 nm / 561 nm

Emission Wavelength: 575 nm

Specificity

Specifically binds to His-tagged proteins without recognizing non-tagged proteins.

Application

Flow Cytometry (Detection the His-tagged protein).

Recommended Dilution

1:50

Isotype Control

The isotype control is sold separately; please refer to Cat. No. DNP-PM1 for product information.

Form

Lyophilized

Formulation

Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4), with trehalose as protectant.

Please contact us for customized product forms or formulations.

Reconstitution

Please refer to the Certificate of Analysis (CoA) for specific instructions.

Shipping

Lyophilized product is shipped at ambient temperature.

Storage

For long term storage, the product should be stored in a lyophilized state at -20°C or lower.

Please protect from light and avoid repeated freeze-thaw cycles.

This product is stable after storage at:
  • -20°C to -70°C for 24 months in lyophilized state;
  • -70°C for 12 months after reconstitution.
  • 2-8 °C for 3 months after reconstitution.
Notices

Product Specific Notices: For research use only.

Background

His tag is a very small molecular weight tag, usually composed of 6-10 histidine (His). It is one of the commonly used tags for protein purification and detection. Due to its small molecular weight, fusion into the target protein has almost no effect on the structure and characteristics of the protein. Anti-his tag antibody can accurately detect, locate and purify His tag fusion protein, so as to provide convenience for the vast number of researchers.