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製品詳細
Application
The Monoclonal Anti-Human IgG Antibody Europium chelate is a universal tool for TR-FRET assays that can bind human IgG or hFc-tagged proteins. This product uses specific anti-human IgG antibody covalently conjugated with Eu³⁺ chelate, and it can be used in combination with other Acceptors directly or indirectly labeled with fluorescent dyes. When the Donor and Acceptor come into close proximity (within a distance of less than 10 nm), a FRET reaction occurs: the 620 nm signal emitted by the Donor upon excitation by a specific light source is received by the Acceptor, which then emits a 665 nm signal.Product Specifications
SourceThis Monoclonal Anti-Human IgG Antibody is a mouse monoclonal antibody recombinantly expressed from Hybridoma.SpecificityThis antibody recognizes all four human IgG isotypes (IgG1, IgG2, IgG3, and IgG4) and multiple engineered variants, including: Human IgG1 (N297A), Human IgG1 (L234A, L235A), Human IgG1 (M252Y, S254T, T256E), Human IgG1 (L234F, L235E, P331S), Human IgG1 (S239D, I332E), Human IgG1 (S298A, E333A, K334A), Human IgG1 (T307A, E380A, N434A), Human IgG2 (A330S, P331S), Human IgG2 (P331S), Human IgG4 (S108P), and Human IgG4 (S228P, L235E).Cross-ReactivityWeak cross-reactivity with cynomolgus IgG, whereas no reactivity to human IgM, human IgA, mouse IgG isotypes (IgG1, IgG2a, IgG2b) or rabbit IgG.FormulationLyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.Physical AppearancePowderStorage (unopened)-20°C to -70°CStorage (opened)-70°CUnit Size1000 tests & 5000 tests & 20000 testsTechnologyTR-FRETIntended UseFor binding the human IgG antibody or human Fc tagged protein as donor in TR-FRET AssayReconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
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Bioactivity-TR-FRET
Please refer to DS document for the assay protocol.
In this TR-FRET assay, Monoclonal Anti-Human IgG Antibody Europium chelate (Cat. No. FRT-TD02) is used as the Donor and Streptavidin Protein FA-Labeled (Cat. No. FRT-TA01) is used as the Acceptor. Biotinylated Human PD-1 Protein, Avitag,His Tag (Cat. No. PD1-H82E4), at 16 ng/well, can bind to the Monoclonal Anti-Human PD-1 Antibody, Human IgG4 with a linear detection range of 10-0.02 μg/mL (QC tested).
In this TR-FRET assay, Monoclonal Anti-Human IgG Antibody Europium chelate (for TR-FRET) (Cat. No. FRT-TD02) is used as the Donor and Monoclonal Anti-His Tag Antibody, Rabbit IgG FA-Labeled (for TR-FRET) (Cat. No. FRT-TA11) is used as the Acceptor. Human Her2, His Tag (Cat. No. HE2-H5225), at 1.25 ng/well, can bind to the Disitamab with a linear detection range of 2-0.002 μg/mL (Routinely tested).
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Can lyophilized proteins remain stable during room-temperature shipping or temporary room-temperature exposure?
In general, lyophilized proteins remain stable during room-temperature shipping or temporary exposure to room-temperature conditions.
Many ACROBiosystems recombinant proteins are supplied in lyophilized (freeze-dried) form. The lyophilization process effectively removes moisture from the product, helping reduce degradation-related reactions and improve protein stability during transportation and storage.
To evaluate the stability of lyophilized proteins under normal temperature conditions, ACROBiosystems conducted a stability study on 11 representative lyophilized protein products at 37°C. The study demonstrated that these proteins maintained good quality stability after storage at 37°C for approximately 20 days. Based on these validation results, temporary room-temperature exposure during shipping or handling is generally not expected to have a significant impact on product quality or downstream application performance.
To ensure optimal long-term stability, products should be stored according to the storage conditions specified in the product Certificate of Analysis (COA) upon receipt.
Supporting Document
最新の進展
医薬品開発の進捗- 英語名：
Immunoglobulin G
- カテゴリ：
- 市販の医薬品数：
1 詳細
- 治験医薬品数：
7 詳細
- 最新の開発ステージ：
Approved
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