Recombinant Monoclonal Anti-Ki-67 Antibody, Rabbit (7A4)

製品詳細

Background
Recombinant Monoclonal Anti-Ki-67 Antibody, Rabbit (7A4) is a Ki-67 primary antibody for IHC application. Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly . Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface. Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility.

Host Species	Rabbit
Isotype	Rabbit IgG, Rabbit Kappa
Clone	7A4
Application	IHC-P
Dilution Ratio	1: 200 - 1: 1000
Form	Liquid
Clonality	Monoclonal
Reactivity	Human
Synonyms	PPP1R105, MIB-1, ki-67
Concentration	0.9~1.1 mg/ml
Specificity	React with Ki-67 protein on the intracellular area of target cells
Purification Tech	Protein A
Preservation	Tris buffer, 1% BSA

Experiment Protocol
LEICA Experiment Protocol
Storage
Shipped at 4°C, Blue Ice. Store at +4°C for short term (1-2 weeks). Store at -20°C for long term.
Avoid freeze / thaw cycle. Upon delivery aliquot.
ACRO Quality Management System

Sample Staining Data
Immunohistochemical analysis of paraffin embedded human cancer tissue labelled with HGS-S239 at 1:500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.