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Product Details
Source
Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 (S1N-M130) is a chimeric monoclonal antibody combining the constant domains of the human IgG1 molecule with mouse variable regions. The variable region was obtained from a mouse immunized with purified recombinant SARS-CoV-2 Spike S1 Protein. This antibody can broadly recognize all Variants of Concerns (VOCs), including Alpha (B.1.1.7), Beta (B.1.351), Gamma (P.1), Delta (B.1.617.2) and Omicron (B.1.1.529).
Isotype
Human IgG1, Kappa
Reactivity
Virus
Specificity
This product is a specific antibody against SARS-CoV-2 Spike protein RBD domain. No cross-reactivity is detected with Spike protein RBD domain of other coronaviruses, including SARS-CoV, MERS-CoV, HCoV-229E, HCoV-NL63, HCoV-OC43 and HCoV-HKU1.
Purification
Protein A purified / Protein G purified.
Concentration
Please refer to the Certificate of Analysis (CoA).
Form
Lyophilized
Formulation
Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4), with trehalose as protectant.
Please contact us for customized product forms or formulations.
Reconstitution
Please refer to the Certificate of Analysis (CoA) for specific instructions.
Shipping
Lyophilized product is shipped at ambient temperature.
Storage
For long term storage, the product should be stored in a lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Notices
Product Specific Notices: For research use only.
ACRO Quality Management System
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Performance Data
Bioactivity-ELISA
Immobilized SARS-CoV-2 S1 protein, His Tag (Cat. No. S1N-C52H2) at 2 μg/mL (100 μL/well) can bind Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 (Cat. No. S1N-M130) with a linear range of 0.2-3 ng/mL (QC tested).
Protocol
Immobilized Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 (S1N-M130) at 1 μg/mL (100 μL/well) can bind SARS-CoV-2 Spike Trimer, His Tag (B.1.1.529/Omicron) (Cat.No. SPN-C52Hz) with a linear range of 0.04-0.125 μg/mL (Routinely tested).
Protocol
Immobilized Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 (S1N-M130) at 1μg/mL (100 μL/well) can bind SARS-CoV-2 Spike RBD, His Tag (B.1.1.529/Omicron) (Cat.No. SPD-C522e) with a linear range of 0.0005-0.004 μg/mL (Routinely tested).
Protocol
Bioactivity-BLI
Loaded Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 (Cat. No. S1N-M130) on AHC Biosensor, can bind SARS-CoV-2 S protein RBD, His Tag (Cat. No. SPD-C52H3) with an affinity constant of 0.603 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Loaded Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 (Cat. No. S1N-M130) on AHC Biosensor, can bind SARS-CoV-2 S protein RBD (N501Y), His Tag (Cat. No. SPD-C52Hn) with an affinity constant of 1.38 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Loaded Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 (Cat. No. S1N-M130) on AHC Biosensor, can bind SARS-CoV-2 S protein RBD (K417N, E484K, N501Y), His Tag (Cat. No. SPD-C52Hp) with an affinity constant of 1.10 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Loaded Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 (Cat. No. S1N-M130) on AHC Biosensor, can bind SARS-CoV-2 S protein RBD (K417T, E484K, N501Y), His Tag (Cat. No. SPD-C52Hr) with an affinity constant of 1.07 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Loaded Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 (Cat. No. S1N-M130) on AHC Biosensor, can bind SARS-CoV-2 Spike RBD (L452R, T478K), His Tag (Cat. No. SPD-C52Hh) with an affinity constant of 1.03 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Loaded Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 (Cat. No. S1N-M130) on AHC Biosensor, can bind SARS-CoV-2 Spike RBD, His Tag (BA.2/Omicron) (Cat. No. SPD-C522g) with an affinity constant of 1.04 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Customer Reviews Writing Reviews
M***g2022-04-16
LikesOrdered AM130 as a binding control for ACE2 competition experiments with a panel of SARS-CoV-2 variant RBDs. This antibody worked incredibly well, showing strong binding (BLI) against all variants tested. As mentioned in the description, this is not a neutralizing antibody and it does not block ACE2, which made it a perfect binding control to ensure that the RBDs were in good shape and functional. Overall would highly recommend this product.
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