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Items | Details |
Detection Method | Chemiluminescence |
Product Type | Magnetic Beads (Anti-His Tag Antibody) |
Quantity Size | 10mg / 100mg |
Physical Appearance | lyophilized powder mixture |
Particle size | 2.8µm |
Beads Surface | Hydrophilic |
Binding Capacity | 3-8μg His tag proteins / mg beads |
Emission Wavelength | Measured relative light units (RLU) at 430 nm |
The Anti-His Tag-coupled Magnetic Beads are 2.8 µm superparamagnetic particles covalently coupled to a highly affinity Monoclonal Mouse Anti-His Tag antibody. The beads can be used to capture the proteins containing N-terminal or C-terminal His Tags (6×His-10×His) in Chemiluminescence procedures.
The Monoclonal Mouse Anti-His Tag antibodies are produced from a hybridoma resulting from fusion of SP2/0 myeloma and B-lymphocytes obtained from a mouse immunized with His Tag Protein. The Anti-His Tag-coupled Magnetic Beads is easy to capture his Tagged protein or other molecules, this ready to use products could greatly save your protein coupling time and hassle, and help us get the best performance and highly reproducible results.
The Anti-His Tag-coupled Magnetic Beads is used to capture his Tagged protein or molecules, it can combination with Acridinium ester markers in chemiluminescence technology, The Acridinium ester markers such as Streptavidin-Acridinium ester can capture the biotinylated proteins or molecules, AHG-Acridinium ester can capture human antibodies, this allows detection of ligand / acceptor binding assay, antigen/antibody binding assay, or antibody screening.
See Certificate of Analysis (CoA) for detailed instruction.
Upon receipt, please store the Beads at -20℃. The shelf life is 1 year at -20 ℃.
Please avoid more than 3 freeze-thaw cycles once reconstitution, immediate use after reconstitution is highly recommended.